RTCB Antibody

Datasheet

Citations (2)

Code	CSB-PA897546LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: NIH/3T3 whole cell lysate, HEK293 whole cell lysate All lanes: RTCB antibody at 3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 56 kDa Observed band size: 56 kDa Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA897546LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human glioma using CSB-PA897546LA01HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA897546LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
Citations
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) RTCB Polyclonal antibody

Uniprot No.

Q9Y3I0

Target Names

RTCB

Alternative Names

Ankyrin repeat domain 54 antibody; Chromosome 22 open reading frame 28 antibody; DJ149A16.6 antibody; HSPC117 antibody; Hypothetical protein LOC51493 antibody; Novel protein HSPC117 antibody; OTTHUMP00000028639 antibody; RP1-149A16.6 antibody; rtcB antibody; RTCB_HUMAN antibody; tRNA-splicing ligase RtcB homolog antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human tRNA-splicing ligase RtcB homolog protein (52-229AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The RTCB Antibody (Product code: CSB-PA897546LA01HU) is Non-conjugated. For RTCB Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA897546LB01HU	RTCB Antibody, HRP conjugated	ELISA
FITC	CSB-PA897546LC01HU	RTCB Antibody, FITC conjugated
Biotin	CSB-PA897546LD01HU	RTCB Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Mammalian IRE1α dynamically and functionally coalesces with stress granules S Liu,Nature cell biology,2024
System-wide Profiling of RNA-Binding Proteins Uncovers Key Regulators of Virus Infection Garcia-Moreno M, et al,Molecular Cell,2019

Customer Reviews and Q&A

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Target Background

Function

Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.

Gene References into Functions

This paper presents a homology model of human RtcB (hRtcB). The structure was analyzed in terms of stereochemical quality, folding reliability, secondary structure, druggability of the active site binding pocket and its metal-binding microenvironment. PMID: 28707320
RTCB, the catalytic subunit of the tRNA ligase complex, and its co-factor archease mediate XBP1 mRNA splicing both in vitro and in vivo. PMID: 25378478
hCLE/C14orf166 associates with DDX1, HSPC117, and FAM98B in a novel transcription-dependent shuttling RNA-transporting complex. PMID: 24608264
Over-expression of HSPC117 mRNA reduced MMP 2 and MMP 14 mRNA expression, while TIMP 2 mRNA expression was up-regulated. PMID: 24941254
study identified HSPC117, a member of the UPF0027 (RtcB) family, as the essential subunit of a tRNA ligase complex; RNA interference-mediated depletion of HSPC117 inhibited maturation of intron-containing pre-tRNA both in vitro and in living cells PMID: 21311021

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Subcellular Location

Nucleus. Cytoplasm.

Protein Families

RtcB family

Database Links

HGNC: 26935

OMIM: 613901

KEGG: hsa:51493

STRING: 9606.ENSP00000216038

UniGene: Hs.474643

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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