RTCB Antibody

Code CSB-PA897546LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: NIH/3T3 whole cell lysate, HEK293 whole cell lysate
    All lanes: RTCB antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 56 kDa
    Observed band size: 56 kDa

  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA897546LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human glioma using CSB-PA897546LA01HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA897546LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) RTCB Polyclonal antibody
Uniprot No.
Target Names
RTCB
Alternative Names
Ankyrin repeat domain 54 antibody; Chromosome 22 open reading frame 28 antibody; DJ149A16.6 antibody; HSPC117 antibody; Hypothetical protein LOC51493 antibody; Novel protein HSPC117 antibody; OTTHUMP00000028639 antibody; RP1-149A16.6 antibody; rtcB antibody; RTCB_HUMAN antibody; tRNA-splicing ligase RtcB homolog antibody
Raised in
Rabbit
Species Reactivity
Human, Mouse
Immunogen
Recombinant Human tRNA-splicing ligase RtcB homolog protein (52-229AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The RTCB Antibody (Product code: CSB-PA897546LA01HU) is Non-conjugated. For RTCB Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA897546LB01HU RTCB Antibody, HRP conjugated ELISA
FITC CSB-PA897546LC01HU RTCB Antibody, FITC conjugated
Biotin CSB-PA897546LD01HU RTCB Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function
Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
Gene References into Functions
  1. This paper presents a homology model of human RtcB (hRtcB). The structure was analyzed in terms of stereochemical quality, folding reliability, secondary structure, druggability of the active site binding pocket and its metal-binding microenvironment. PMID: 28707320
  2. RTCB, the catalytic subunit of the tRNA ligase complex, and its co-factor archease mediate XBP1 mRNA splicing both in vitro and in vivo. PMID: 25378478
  3. hCLE/C14orf166 associates with DDX1, HSPC117, and FAM98B in a novel transcription-dependent shuttling RNA-transporting complex. PMID: 24608264
  4. Over-expression of HSPC117 mRNA reduced MMP 2 and MMP 14 mRNA expression, while TIMP 2 mRNA expression was up-regulated. PMID: 24941254
  5. study identified HSPC117, a member of the UPF0027 (RtcB) family, as the essential subunit of a tRNA ligase complex; RNA interference-mediated depletion of HSPC117 inhibited maturation of intron-containing pre-tRNA both in vitro and in living cells PMID: 21311021

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Subcellular Location
Nucleus. Cytoplasm.
Protein Families
RtcB family
Database Links

HGNC: 26935

OMIM: 613901

KEGG: hsa:51493

STRING: 9606.ENSP00000216038

UniGene: Hs.474643

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