RTCB Antibody

Datasheet
Code CSB-PA897546LA01HU
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Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. Q9Y3I0
Image
  • Western Blot
    Positive WB detected in: NIH/3T3 whole cell lysate, HEK293 whole cell lysate
    All lanes: RTCB antibody at 3μg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 56 kDa
    Observed band size: 56 kDa

  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA897546LA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human glioma using CSB-PA897546LA01HU at dilution of 1:100

  • Immunofluorescent analysis of Hela cells using CSB-PA897546LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

Immunogen Recombinant Human tRNA-splicing ligase RtcB homolog protein (52-229AA)
Raised in Rabbit
Species Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:1000-1:5000, IHC:1:20-1:200, IF:1:50-1:200
Relevance Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias tRNA-splicing ligase RtcB homolog (EC 6.5.1.3), RTCB, C22orf28
Immunogen Species Human
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Cell Biology
Target Names RTCB
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
References
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Function Catalytic subunit of the tRNA-splicing ligase complex that acts by directly joining spliced tRNA halves to mature-sized tRNAs by incorporating the precursor-derived splice junction phosphate into the mature tRNA as a canonical 3',5'-phosphodiester. May act as an RNA ligase with broad substrate specificity, and may function toward other RNAs.
Subcellular Location Nucleus, Cytoplasm
Protein Families RtcB family
Database Links

HGNC: 26935

OMIM: 613901

KEGG: hsa:51493

STRING: 9606.ENSP00000216038

UniGene: Hs.474643

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