SEMA5B Antibody

Datasheet

Code	CSB-PA868389LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse heart tissue All lanes: SEMA5B antibody at 1μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 126, 120, 124, 132 kDa Observed band size: 126 kDa Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA868389LA01HU at dilution of 1:100 Immunohistochemistry of paraffin-embedded human lung tissue using CSB-PA868389LA01HU at dilution of 1:100 Immunofluorescence staining of Hela cells with CSB-PA868389LA01HU at 1:33, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SEMA5B Polyclonal antibody

Uniprot No.

Q9P283

Target Names

SEMA5B

Alternative Names

FLJ10372 antibody; KIAA1445 antibody; SEM5B_HUMAN antibody; SEMA5B antibody; SEMAG antibody; Semaphorin-5B antibody; SemG antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse

Immunogen

Recombinant Human Semaphorin-5B protein (845-1013AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SEMA5B Antibody (Product code: CSB-PA868389LA01HU) is Non-conjugated. For SEMA5B Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA868389LB01HU	SEMA5B Antibody, HRP conjugated	ELISA
FITC	CSB-PA868389LC01HU	SEMA5B Antibody, FITC conjugated
Biotin	CSB-PA868389LD01HU	SEMA5B Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:20-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

May act as positive axonal guidance cues.

Gene References into Functions

SEMA5B could possibly serve as a candidate gene for alterations associated with asbestos exposure. PMID: 26463840
Sema5B regulates the development and maintenance of synapse size and number in hippocampal neurons. PMID: 19463192

Subcellular Location

Membrane; Single-pass type III membrane protein.

Protein Families

Semaphorin family

Database Links

HGNC: 10737

OMIM: 609298

KEGG: hsa:54437

STRING: 9606.ENSP00000350215

UniGene: Hs.210870

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Email: [email protected]
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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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