SETD3 Antibody

Datasheet

Code	CSB-PA769772LA01HU
Size	US$166
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Image	IHC image of CSB-PA769772LA01HU diluted at 1:400 and staining in paraffin-embedded human appendix tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA769772LA01HU diluted at 1:400 and staining in paraffin-embedded human prostate cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SETD3 Polyclonal antibody

Uniprot No.

Q86TU7

Target Names

SETD3

Alternative Names

C14orf154 antibody; Chromosome 14 open reading frame 154 antibody; DKFZp761E1415 antibody; FLJ23027 antibody; Histone lysine N methyltransferase setd3 antibody; Histone-lysine N-methyltransferase setd3 antibody; MGC87236 antibody; SET domain containing 3 antibody; SET domain containing protein 3 antibody; SET domain-containing protein 3 antibody; SETD 3 antibody; Setd3 antibody; SETD3_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Histone-lysine N-methyltransferase setd3 protein (166-265AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SETD3 Antibody (Product code: CSB-PA769772LA01HU) is Non-conjugated. For SETD3 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA769772LB01HU	SETD3 Antibody, HRP conjugated	ELISA
FITC	CSB-PA769772LC01HU	SETD3 Antibody, FITC conjugated
Biotin	CSB-PA769772LD01HU	SETD3 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Protein-histidine N-methyltransferase that specifically mediates 3-methylhistidine (tele-methylhistidine) methylation of actin at 'His-73'. Histidine methylation of actin is required for smooth muscle contraction of the laboring uterus during delivery. Does not have protein-lysine N-methyltransferase activity and probably only catalyzes histidine methylation of actin.

Gene References into Functions

data reveal a new SETD3-dependent methylation-based signaling pathway at chromatin that regulates VEGF expression under normoxic and hypoxic conditions. PMID: 27845446
these data elucidated that a GSK3beta-FBXW7beta-dependent mechanism controls SETD3 protein levels during the cell cycle and attenuates its oncogenic role in liver tumorigenesis. PMID: 28442573
MAF1, RNF7 and SETD3 are identified as PCNA-associated proteins in human cells and given this interaction with PCNA, Maf1, RNF7, and SetD3 are potentially involved in DNA replication, DNA repair, or associated processes. PMID: 26030842

Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Class V-like SAM-binding methyltransferase superfamily, Histone-lysine methyltransferase family, SETD3 subfamily

Database Links

HGNC: 20493

OMIM: 615671

KEGG: hsa:84193

STRING: 9606.ENSP00000327436

UniGene: Hs.510407

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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