SLC22A25 Antibody

Datasheet

Code	CSB-PA740875LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Hela whole cell lysate All lanes: SLC22A25 antibody at 6.8μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 62 kDa Observed band size: 62 kDa IHC image of CSB-PA740875LA01HU diluted at 1:300 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA740875LA01HU at 1:100, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SLC22A25 Polyclonal antibody

Uniprot No.

Q6T423

Target Names

SLC22A25

Alternative Names

HIMTP antibody; MGC120420 antibody; Organic anion transporter UST6 antibody; S22AP_HUMAN antibody; SLC22A25 antibody; Solute carrier family 22 member 25 antibody; UST6 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Solute carrier family 22 member 25 protein (31-145AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The SLC22A25 Antibody (Product code: CSB-PA740875LA01HU) is Non-conjugated. For SLC22A25 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA740875LB01HU	SLC22A25 Antibody, HRP conjugated	ELISA
FITC	CSB-PA740875LC01HU	SLC22A25 Antibody, FITC conjugated
Biotin	CSB-PA740875LD01HU	SLC22A25 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:100-1:300
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Gene References into Functions

TNFSF13, SPATC1L, SLC22A25 and SALL4 may thus be novel susceptibility loci for atrial fibrillation in the Japanese population PMID: 28849223
cloning of a novel human slc22 family member, UST6, expressed exclusively in liver in both embryo and adult, is reported [UST6] PMID: 15054140
Review summarizes current knowledge on the functional and phenotypic consequences of genetic variation in intestinally, hepatically and renally expressed members of solute carrier family (SLC) member 25. PMID: 18466105

Subcellular Location

Membrane; Multi-pass membrane protein.

Protein Families

Major facilitator (TC 2.A.1) superfamily, Organic cation transporter (TC 2.A.1.19) family

Tissue Specificity

Expressed exclusively in liver in both embryo and adult.

Database Links

HGNC: 32935

OMIM: 610792

KEGG: hsa:387601

STRING: 9606.ENSP00000307443

UniGene: Hs.332119

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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