Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
All lanes: SWI/SNF complex subunit SMARCC2 antibody at 2ug/ml+mouse liver tissue
Goat polyclonal to rabbit at 1/10000 dilution
Predicted band size: 133,125,127 kDa
Observed band size: 133 kDa
Immunohistochemistry of paraffin-embedded human rectal cancer using CSB-PA851527ESR2HU at dilution 1:100
Immunohistochemistry of paraffin-embedded human epityphlon using CSB-PA851527ESR2HU at dilution 1:100
Immunohistochemistry of paraffin-embedded human tonsil using CSB-PA851527ESR2HU at dilution 1:100
Chromatin Immunoprecipitation Hela(1.1*106)were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4ug anti-SMARCC2 or a control normal rabbit IgG . The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP851527HU) against the ESR1 pS2 promoter.
Recombinant human SWI/SNF complex subunit SMARCC2 protein (300-650AA)
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth
Storage Buffer :
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification method :
Antigen Affinity Purified
BRG1-associated factor 170,BAF170,SWI/SNF complex 170 kDa subunit,SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2,SMARCC2,BAF170