SMARCC2 Antibody

Datasheet

Code CSB-PA851527ESR2HU
Size US$167Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. Q8TAQ2
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Image
  • Immunohistochemistry of paraffin-embedded human colon cancer using CSB-PA851527ESR2HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human epityphlon tissue using CSB-PA851527ESR2HU at dilution of 1:100

  • Chromatin Immunoprecipitation Hela (1.1*106) were cross-linked with formaldehyde, sonicated, and immunoprecipitated with 4µg anti-SMARCC2 or a control normal rabbit IgG. The resulting ChIP DNA was quantified using real-time PCR with primers (CSB-PP851527HU) against the ESR1 pS2 promoter.

Immunogen Recombinant Human SWI/SNF complex subunit SMARCC2 protein (300-650AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, IHC, ChIP; Recommended dilution: IHC:1:20-1:200
Relevance Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Can stimulate the ATPase activity of the catalytic subunit of these complexes. May be required for CoREST dependent repression of neuronal specific gene promoters in non-neuronal cells. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth
Form Liquid
Conjugate Non-conjugated
Storage Buffer PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias SWI/SNF complex subunit SMARCC2 (BRG1-associated factor 170) (BAF170) (SWI/SNF complex 170 kDa subunit) (SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily C member 2), SMARCC2, BAF170
Immunogen Species Human
Protocols ELISA Protocol
Immunohistochemistry (IHC) Protocol
Native Chromatin Immunoprecipitation(ChIP) Protocol
Research Area Epigenetics and Nuclear Signaling
Target Names SMARCC2
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Function Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner
Subcellular Location Nucleus
Protein Families SMARCC family
Tissue Specificity Ubiquitously expressed.
Database Links

HGNC: 11105

OMIM: 601734

KEGG: hsa:6601

STRING: 9606.ENSP00000267064

UniGene: Hs.236030

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