SMPD2 Antibody

Code CSB-PA021846LA01HU
Size US$166
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Image
  • IHC image of CSB-PA021846LA01HU diluted at 1:200 and staining in paraffin-embedded human endometrial cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA021846LA01HU diluted at 1:200 and staining in paraffin-embedded human pancreatic tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA021846LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) SMPD2 Polyclonal antibody
Uniprot No.
Target Names
SMPD2
Alternative Names
SMPD2; Sphingomyelin phosphodiesterase 2; Lyso-platelet-activating factor-phospholipase C; Lyso-PAF-PLC; Neutral sphingomyelinase; N-SMase; nSMase; nSMase1
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Sphingomyelin phosphodiesterase 2 protein (199-301AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The SMPD2 Antibody (Product code: CSB-PA021846LA01HU) is Non-conjugated. For SMPD2 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA021846LB01HU SMPD2 Antibody, HRP conjugated ELISA
FITC CSB-PA021846LC01HU SMPD2 Antibody, FITC conjugated
Biotin CSB-PA021846LD01HU SMPD2 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, IHC, IF
Recommended Dilution
Application Recommended Dilution
IHC 1:200-1:500
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Customer Reviews and Q&A

 Customer Reviews
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Applications : Fluorescent Western Blotting

Sample type: cells

Review: SMPD2, neutral sphingomyelinase.

By Anonymous

Target Background

Function
Catalyzes the hydrolysis of sphingomyelin to form ceramide and phosphocholine. Ceramide mediates numerous cellular functions, such as apoptosis and growth arrest, and is capable of regulating these 2 cellular events independently. Also hydrolyzes sphingosylphosphocholine. Hydrolyze 1-acyl-2-lyso-sn-glycero-3-phosphocholine (lyso-PC) and 1-O-alkyl-2-lyso-sn-glycero-3-phosphocholine (lyso-platelet-activating factor).
Gene References into Functions
  1. Interestingly, neutral but not acid sphingomyelinase was mandatory for PorBIA -mediated Neisseria gonorrhoeae invasion into host cells. PMID: 25224994
  2. nSMase1 (sphingomyelin phosphodiesterase 2, SMPD2)has a common central role in ceramide signaling during the stress and cytokine responses and apoptosis. PMID: 25168245
  3. These data suggest that Ox-LDL activates N-SMase-ceramide signaling pathway, and stimulates phosphorylation of p38 MAPK, leading to apoptosis in vascular smooth muscle cells, which initiates VSMC calcification. PMID: 24358176
  4. NSM2 plays a key role in the cellular response to hyperosmolar stress, and its activity regulates both cytokine secretion and lipid droplet formation. PMID: 22899568
  5. nSMase2 has a role in ceramide generation, aberrant apoptosis, and lung injury under cigarette smoke exposure PMID: 20448054
  6. N-SMase at the cytofacial plasma membrane is an essential element for the proper orientation of PMNs in FMLP gradients, at least in part by polarizing the distribution of Rac 1/2 and RhoA GTPases. PMID: 20378749
  7. Results demonstrate that WithaD enhance the ceramide accumulation by activating N-SMase 2, modulate phosphorylation of the JNK and p38MAPK and induced apoptosis in both myeloid and lymphoid cells along with primary cells derived from leukemia patients. PMID: 20836852
  8. Data show DA remarkably increased the NSMase2 message and protein, whereas little change in NSMase1 and NSMase3 mRNAs. PMID: 19698806
  9. nSMase2 functions as a growth suppressor in MCF7 cells, linking confluence to the G(0)/G(1) cell cycle check point. PMID: 15051724
  10. identification of 15 polymorphisms, including 9 novel SNPs, in the SMPD2 gene; one haplotype showed associations with the extrinsic type of atopic dermatitis subgroup PMID: 17212982
  11. The catalytic region of neutral sphingomyelinase 2 where palmitoylation occurs is found to be localized at the inner leaflet of the plasma membrane. PMID: 17272284
  12. This study underlines the importance of neutral sphingomyelinase-ceramide pathway in mediating oxidative stress-induced apoptosis and cell death of human primary oligodendrocytes. PMID: 18040843

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Subcellular Location
Membrane; Multi-pass membrane protein.
Protein Families
Neutral sphingomyelinase family
Database Links

HGNC: 11121

OMIM: 603498

KEGG: hsa:6610

STRING: 9606.ENSP00000258052

UniGene: Hs.55235

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