SOAT2 Antibody

Datasheet

Code	CSB-PA022386ESR2HU
Size	US$166
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate(60µg), HepG2 whole cell lysate(30µg), HT29 whole cell lysate(60µg), HT29 whole cell lysate(30µg), 293T whole cell lysate(30µg), Hela whole cell lysate(30µg),LO2 whole cell lysate(30µg) All lanes: SOAT2 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 60,58,51,35 kDa Observed band size: 58,51,35 kDa Exposure time: 120s Immunofluorescence staining of HepG2cell with CSB-PA022386ESR2HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of HepG2 cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) SOAT2 Polyclonal antibody

Uniprot No.

O75908

Target Names

SOAT2

Alternative Names

SOAT2 antibody; ACACT2 antibody; ACAT2 antibody; Sterol O-acyltransferase 2 antibody; EC 2.3.1.26 antibody; Acyl-coenzyme A:cholesterol acyltransferase 2 antibody; ACAT-2 antibody; Cholesterol acyltransferase 2 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Sterol O-acyltransferase 2 protein

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:20-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

To produce the SOAT2 polyclonal antibodies, rabbits were immunized with recombinant human Sterol O-acyltransferase 2 protein. This process stimulates the rabbits' immune system to generate a varied population of antibodies that recognize various epitopes on the antigen. The antibodies are then purified from the rabbits' sera by antigen affinity chromatography.

The SOAT2 antibody is recommended for use in ELISA, WB, and IF applications. This SOAT2 polyclonal antibody specifically recognizes and binds to the human SOAT2 protein, facilitating the detection and quantification of SOAT2 expression in cells and tissues.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Catalyzes the formation of fatty acid-cholesterol esters, which are less soluble in membranes than cholesterol. Plays a role in lipoprotein assembly and dietary cholesterol absorption. Utilizes oleoyl-CoA ((9Z)-octadecenoyl-CoA) and linolenoyl-CoA ((9Z,12Z,15Z)-octadecatrienoyl-CoA) as substrates. May provide cholesteryl esters for lipoprotein secretion from hepatocytes and intestinal mucosa.; Has lower enzymatic activity compared to isoform 1.; Has lower enzymatic activity compared to isoform 1.

Gene References into Functions

Describe a gender-related difference in hepatic ACAT2 activity in normolipidemic non-obese Chinese patients suggesting a possible role for ACAT2 in the regulation of cholesterol metabolism in humans. PMID: 19467657
ACAT-2 expression was negative in clear cell type renal cell carcinoma and normal kidney. PMID: 18269457
ACAT2 expression was induced upon R1881 (androgen agonist) treatment in prostate cancer cells PMID: 18000807
HNF4alpha positively regulates ACAT2 gene expression at mRNA level. Overexpression of HNF4alpha increased ACAT2 expression, whereas knockdown of HNF4alpha decreased ACAT2 expression. PMID: 22155889
results show that the enzymatic activity of mutant Glu(14)Gly is approximately two times higher than wildtype, and that this increase is primarily due to the increased expression and/or stability of the mutant ACAT2 protein PMID: 25917363
Low ACAT2 expression is associated with clear cell renal cell carcinoma. PMID: 26715271
data demonstrate that the ACAT2 expression of human leukocytes is responsible for the excretion of lipoproteins containing cholesteryl/steryl esters (CE/SE), and suggest that the excretion of lipoproteins containing the ACAT2-catalyzed CS/SE may avoid cytotoxicity PMID: 27688150
Lipid-induced stabilization of ACAT2 ameliorates lipotoxicity from excessive cholesterol and fatty acid. Cysteine ubiquitylation of ACAT2 constitutes an important mechanism for sensing lipid-overload-induced ROS and fine-tuning lipid homeostasis. PMID: 28604676
human ACAT2 is transcriptionally regulated by cholesterol. PMID: 17950700
TG-interacting factor 1 (Tgif1) is an important repressor of SOAT2 gene expression. PMID: 24478032
CDX2, a known positive regulator of hepatocyte differentiation, was regulated by miR-181d and directly activated SOAT2 gene expression. PMID: 24103759
We found a new single-nucleotide polymorphism (SNP; a point mutation in intron 1, IVS1 -8 G-->C) in the ACAT-2 gene. Our data suggest that the ACAT-2 gene may not affect lipid levels in humans. PMID: 12621162
Fully differentiated macrophages express ACAT2 in addition to ACAT1 under various pathologic conditions. PMID: 14615411
ACAT-1 transcripts predominate in human liver and ACAT-2 transcripts predominate in human duodenum and support the notion that ACAT-2 has an important regulatory role in liver and intestine. PMID: 14729857
increasing DGAT1, ACAT1, or ACAT2 expression stimulates the assembly and secretion of VLDL from liver cells PMID: 15308631
ACAT2 provided the major cholesterol-esterifying activity in 3 of 4 human liver samples. PMID: 15451793
transcription factors hepatic nuclear factor 1 (HNF1)alpha and beta play an important part in the regulation of the ACAT2 promoter PMID: 15961790
The structural features of various sterols as substrates and/or activators of ACAT1 and ACAT2 in vitro are reported. PMID: 15992359
Serum-induced depletion of cellular cholesterol available for esterification by ACAT was a strong, independent predictor of major adverse cardiovascular events and death. PMID: 16230498
Elevated ACAT2 expression may serve as a new biomarker for certain form(s) of hepatocellular carcinoma. PMID: 16274362
Alternative splicing produces two human ACAT2 mRNA variants that encode the novel ACAT2 isoenzymes. Our findings might help to understand the regulation of the ACAT2 gene expression under certain physiological and pathological conditions. PMID: 16331323
histidine residues located at the active site are very crucial both for the catalytic activity of the enzyme and for distinguishing ACAT1 from ACAT2 with respect to enzyme catalysis and substrate specificity PMID: 16647063
We resequenced 4 candidate genes for HDL regulation and identified several functional nonsynonymous mutations including 4 in lecithin:cholesterol acyltransferase (LCAT), leaving 88% (110/124) of HDL deficient subjects without a genetic diagnosis. PMID: 17303779

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Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

Membrane-bound acyltransferase family, Sterol o-acyltransferase subfamily

Tissue Specificity

Expression seems confined in hepatocytes and enterocytes.

Database Links

HGNC: 11178

OMIM: 601311

KEGG: hsa:8435

STRING: 9606.ENSP00000301466

UniGene: Hs.656544

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

Cholesterol metabolism

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