SPAG4 Antibody

Datasheet

Code	CSB-PA989865
Size	US$166
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Image	The image on the left is immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using CSB-PA989865(SPAG4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200) The image on the left is immunohistochemistry of paraffin-embedded Human lung cancer tissue using CSB-PA989865(SPAG4 Antibody) at dilution 1/50, on the right is treated with synthetic peptide. (Original magnification: ×200) Gel: 12%SDS-PAGE, Lysate: 40 μg, Lane 1-2: Hela cells, K562 cells, Primary antibody: CSB-PA989865(SPAG4 Antibody) at dilution 1/1100, Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution, Exposure time: 30 seconds
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Uniprot No.

Q9NPE6

Target Names

SPAG4

Alternative Names

SPAG4; SUN4; Sperm-associated antigen 4 protein; Outer dense fiber-associated protein SPAG4; SUN domain-containing protein 4

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Synthetic peptide of Human SPAG4

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

-20°C, pH7.4 PBS, 0.05% NaN3, 40% Glycerol

Form

Liquid

Tested Applications

ELISA,WB,IHC

Recommended Dilution

Application	Recommended Dilution
ELISA	1:2000-1:10000
WB	1:500-1:2000
IHC	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Involved in spermatogenesis. Required for sperm head formation but not required to establish and maintain general polarity of the sperm head. Required for anchoring and organization of the manchette. Required for targeting of SUN3 and probably SYNE1 through a probable SUN1:SYNE3 LINC complex to the nuclear envelope and involved in accurate posterior sperm head localization of the complex. May anchor SUN3 the nuclear envelope. Involved in maintenance of the nuclear envelope integrity. May assist the organization and assembly of outer dense fibers (ODFs), a specific structure of the sperm tail.

Gene References into Functions

SPAG4, in cooperation with Nesprin3, has a fundamental pathological function in the migration of lung carcinoma cells. PMID: 29901114
SPAG4 knockdown reduces the invasion capability of RCC cells. PMID: 23818324
SPAG4 is an independent prognostic factor in renal cell carcinoma and plays a crucial role in cytokinesis to defend against hypoxia-induced tetraploid formation. PMID: 23602831

Subcellular Location

Membrane; Multi-pass membrane protein. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, flagellum axoneme. Nucleus envelope. Nucleus inner membrane.

Tissue Specificity

Predominantly epressed in testis. Expressed in ejaculated spermatozoa (at protein level).

Database Links

HGNC: 11214

OMIM: 603038

KEGG: hsa:6676

STRING: 9606.ENSP00000363391

UniGene: Hs.123159

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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