Product Details

Uniprot No.

Target Names

SPAG5

Alternative Names

Astrin antibody; Deepest antibody; hMAP 126 antibody; hMAP126 antibody; MAP 126 antibody; MAP126 antibody; Mitotic spindle associated protein antibody; Mitotic spindle associated protein p126 antibody; Mitotic spindle coiled coil related protein antibody; Mitotic spindle-associated protein p126 antibody; SPAG 5 antibody; SPAG5 antibody; SPAG5_HUMAN antibody; Sperm associated antigen 5 antibody; Sperm tail protein Spag 5 antibody; Sperm tail protein Spag5 antibody; Sperm-associated antigen 5 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Human SPAG5

Immunogen Species

Homo sapiens (Human)

Isotype

IgG

Purification Method

Antigen Affinity purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. -20°C, Avoid freeze / thaw cycles.

Tested Applications

ELISA,WB,IF,IP

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol
Immunoprecipitation (IP) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Target Background

Function

Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes. Involved in centriole duplication. Required for CDK5RAP2, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2. In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis. May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU.

Gene References into Functions

we report the differential SPAG5 expression at both the protein and mRNA levels in Hepatocellular Carcinoma. SPAG5 may be considered a new prognostic marker for Hepatocellular Carcinoma patients, and targeting SPAG5 could provide new treatment strategies for Hepatocellular Carcinoma. PMID: 30157168
our data provide a novel evidence for the biological and clinical significance of SPAG5 as a potential biomarker, and we demonstrate that SPAG5-b-catenin-SCARA5 might be a novel pathway involved in hepatocellular carcinoma progression. PMID: 30249289
MSI2 expression is increased in epithelial cells adjacent to breast carcinoma, and increases with increasing proximity. PMID: 29093438
Data show that aurora-B regulates end-on conversion in human cells and indicate a late role for SPAG5 protein (Astrin)-SKAP complex in the end-on conversion process. PMID: 28751710
Low SPAG5 expression is associated with low chemotherapy sensitivity in breast cancer. PMID: 27312051
findings reveal a direct physical link between two important regulators of mitotic progression and demonstrate the critical role of the NuMA-Astrin interaction for accurate cell division. PMID: 27462074
Mechanistically, Plk1 acts as an upstream kinase for Astrin phosphorylation by Cdk1 and targeting phospho-Astrin to KTs, leading to the recruitment of outer KT components, such as Cenp-E, and the stable attachment of spindles to KTs. PMID: 27325694
Results showed a progression-driving role of SPAG5 in prostate cancer which can be regulated by miR-539. PMID: 27037000
Molecular requirements for the inter-subunit interaction and kinetochore recruitment of SKAP and Astrin have been reported. PMID: 27095104
SPAG5 upregulation relates to poor prognosis in cervical cancer patients, and SPAG5 is a regulator of mTOR activity during taxol treatment in cervical cancer. PMID: 24853425
Astrin acts as a mitotic phosphoprotein during the regulation of mitosis. PMID: 25009111
Study identifies astrin as an essential negative mTORC1 regulator in the cellular stress response. Upon stress, astrin inhibits mTORC1 association and recruits the mTORC1 component raptor to stress granules, thereby preventing mTORC1-hyperactivation-induced apoptosis. PMID: 23953116
The rsults of this study the increased expression of this gene may play a pathogenic role durin testicular development in subjects with DS and cryptorchidism. PMID: 22773063
Data show that CLASP1-astrin-Kif2b complex acts as a central switch at kinetochores that defines mitotic progression and promotes fidelity by temporally regulating kinetochore-microtuble attachments. PMID: 20852589
These findings suggest that the silencing of astrin induce a p53-dependent apoptosis and has an additive effect on paclitaxel treatment. PMID: 16546135
a model is proposed wherein hNinein regulates the dynamic movement of Astrin throughout the cell cycle and this interaction, in turn, is required for maintenance of centrosome/spindle pole integrity. PMID: 17383637
Results report that depletion of the microtubule and kinetochore protein astrin results in checkpoint-arrested cells with multipolar spindles and separated sister chromatids, which is consistent with untimely separase activation. PMID: 17664331
GSK3beta interacts with and phosphorylates the spindle-associated protein Astrin, resulting in targeting Astrin to the spindle microtubules and kinetochores. PMID: 18055457
Astrin acts upstream of Aurora-A to regulate its mitotic spindle localization. PMID: 18361916
Results suggest that SNM1B/Apollo and Astrin function together to enforce the prophase checkpoint in response to spindle stress. PMID: 19197158
hMap126 is abundantly expressed in testis and localized in mitotic spindle and may be involved in the functional and dynamic regulation of mitotic spindles. PMID: 11549262

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Subcellular Location

Cytoplasm. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Cytoplasm, cytoskeleton, spindle pole. Chromosome, centromere, kinetochore. Midbody. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Cytoplasmic granule. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriolar satellite.

Tissue Specificity

Highly expressed in testis. Detected at low levels in placenta, liver, pancreas, thymus and colon.

Database Links

HGNC: 13452

OMIM: 615562

KEGG: hsa:10615

STRING: 9606.ENSP00000323300

UniGene: Hs.514033