TFAM Antibody

Code CSB-PA023413LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western blot
    All lanes: TFAM antibody at 8µg/ml
    Lane 1: Jurkat whole cell lysate
    Lane 2: MCF-7 whole cell lysate
    Lane 3: A431 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 30, 26 kDa
    Observed band size: 26 kDa

  • Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA023413LA01HU at dilution of 1:100

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) TFAM Polyclonal antibody
Uniprot No. Q00059
Target Names TFAM
Alternative Names anscription factor 6-like 1 antibody; Mitochondrial transcription factor 1 antibody; mitochondrial transcription factor A antibody; MtTF1 antibody; mtTFA antibody; TCF 6 antibody; TCF-6 antibody; TCF6 antibody; TCF6L1 antibody; TCF6L2 antibody; TCF6L3 antibody; TFAM antibody; TFAM_HUMAN antibody; Transcription factor 6 antibody; Transcription factor 6 like 2 (mitochondrial transcription factor) antibody; Transcription factor 6 like 2 antibody; Transcription factor 6-like 2 antibody; transcription factor 6-like 3 antibody; Transcription factor A, mitochondrial antibody; Transcription factor A, mitochondrial antibody; Transcription factor A, mitochondrial precursor antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Transcription factor A, mitochondrial protein (43-246AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Citations

Concurrent EPA and DHA Supplementation Impairs Brown Adipogenesis of C2C12 Cells. S Ghnaimawi,Frontiers in Genetics,2020

Applications: Western Blot
Antibody dilution factor: 1:1000
Review: Protein level measurement by western blot of brown adipocytes signature proteins and GAPDH as housekeeping gene from C2C12 7 days after induction of brown adipogenesis in the absence (CON) or presence of 50 µM EPA and 50 µM DHA (FA) n = 4.
PMID: 32595696

Target Data

Function Binds to the mitochondrial light strand promoter and functions in mitochondrial transcription regulation. Required for accurate and efficient promoter recognition by the mitochondrial RNA polymerase. Promotes transcription initiation from the HSP1 and the light strand promoter by binding immediately upstream of transcriptional start sites. Is able to unwind DNA. Bends the mitochondrial light strand promoter DNA into a U-turn shape via its HMG boxes. Required for maintenance of normal levels of mitochondrial DNA. May play a role in organizing and compacting mitochondrial DNA.
Gene References into Functions
  1. Study shows that the human mitochondrial transcription factor A (TFAM) is a versatile G-quadruplex (G4s) binding protein. These TFAM-G4 interactions suggest functional recognition of G4s in the mitochondria. PMID: 28276514
  2. CTGF can reduce glycolysis and mitochondrial OXPHOS pathways by increasing mtTFA protein degradation and in turn reduces cancer migration and invasion in oral squamous cell carcinoma (OSCC). PMID: 28438434
  3. radiation stimulated the levels of TFAM mRNA and protein. PMID: 29856906
  4. Two tag SNPs of TFAM and POLG were associated with multibacillary leprosy in Han Chinese from Southwest China. PMID: 28958595
  5. Data revealed that TFAM expression level was regulated by TP73-AS1/miR-200a axis in breast cancer cells. PMID: 28639399
  6. Study clearly shows that hTFAM efficiently breaks the mitochondria-mediated vicious cycle in both Alzheimer's disease (AD) model neurons and mouse brains, resulting in an effective improvement of the AD pathophysiology including Abeta accumulation and cognitive dysfunction. PMID: 27897204
  7. these findings establish a new link between HIF-2alpha and MAPK-signaling that mediates the adaptive regulation of mitochondrial gene expression under low oxygen tension. PMID: 28709643
  8. the results presented in this study obviously provided the evidence that TFAM was upregulated in glioma cell line and glioma tissue specimens. Therefore TFAM may be a novel diagnostic marker and therapeutic target for glioma and other cancer. PMID: 28440425
  9. Biotinylated TMP interacts with TFAM. PMID: 28465355
  10. TFAM polymorphisms (rs1937, rs1049432, and rs11006132) as well as their haplotypes did not show significant association with aggressiveness of prostate cancer in overweight or obese men. PMID: 27843028
  11. results suggest a nucleation-cooperativity-based mechanism for sensitive detection of mitochondrial DNA and pathogen genomes, and identify HMGB/TFAM proteins as DNA-structuring host factors; they provide an explanation for the peculiar cGAS dimer structure and suggest that cGAS preferentially binds incomplete nucleoid-like structures or bent DNA PMID: 28902841
  12. p53 might incrase mtDNA copy number through its regulation on TFAM expression via TFAMpromoter. PMID: 27732955
  13. TFAM is a gene regulator that acts to mitigate calcium mishandling and ROS production by wrapping around mitochondrial DNA complexes. TFAM's regulatory functions over serca2a, NFAT, and Lon protease contribute to cardiomyocyte stability. [review] PMID: 27166683
  14. The results suggest that a high mtTFA expression is a useful marker for tumor progression and a poor prognosis in left-sided colorectal cancer patients. PMID: 29277826
  15. Study reports crystal structures of human mitochondrial transcription initiation complexes assembled on both light and heavy strand promoters. The structures reveal how transcription factors TFAM and TFB2M assist mitochondrial RNA polymerase to achieve promoter-dependent initiation. PMID: 29149603
  16. TFAM is essential for transcription, replication and packaging of mtDNA into nucleoids. Tfam knockout mice display embryonic lethality secondary to severe mtDNA depletion. In this report, for the first time, we associate a homozygous variant in TFAM with a novel mtDNA depletion syndrome. PMID: 27448789
  17. Tissue microarray (TMA) data showed that elevated expression of TFAM was related to the histological grade and TNM stage of NSCLC patients. PMID: 26820294
  18. The authors did not find statistically significant differences in the genotype frequencies for the TFAM +35G/C polymorphism between women with polycystic ovary syndrome and controls and found that mtDNA copy number is not associated with TFAM+35G/C SNP in polycystic ovary syndrome patients. PMID: 29030253
  19. Alleles in mitochondrial transcription factor A (TFAM) and AP endonuclease 1 (APE1) are associated with reduced cognitive performance. PMID: 28242328
  20. In vivo, AICAR significantly reduced osteosarcoma growth without apparent body weight loss and AICAR increased both mitochondrial proliferation and apoptotic activity in treated tumor tissues. AICAR showed anticancer effects in osteosarcoma cells through an AMPK-dependent peroxisome proliferatoractivated receptor-gamma coactivator-1alpha (PGC-1alpha)/mitochondrial transcription factor A (TFAM)/mitochondrial pathway PMID: 27878239
  21. the present study identified the interaction of miR-590-3p and TFAM in colon cancer. TFAM was identified as a target of miR-590-3p, and miR-590-3p enhanced the proliferation of SW480 cells. PMID: 27878255
  22. The results of the present study provided evidence that resistance to cisplatin chemotherapy in ERpositive breast cancer may be through TFAM and indicated that TFAM may be a target for chemoresistance in patients with breast cancer. PMID: 27779689
  23. miR-199a-3p is able to attenuate cisplatin resistance in breast cancer cells through inhibiting TFAM expression. PMID: 28126676
  24. We conducted a meta-analysis of studies involving CHAT, TFAM, and VR22 polymorphisms and Alzheimer disease susceptibility. For TFAM and VR22, no significant association was detected in studied single-nucleotide polymorphisms (SNPs).Rs1937 and rs2306604 of TFAM, are not significantly associated with AD risk. PMID: 27272392
  25. Results showed that TFAM formed oligomers in mitochondria by in situ chemical cross-linking and suggested the importance of the dimerization of TFAM molecules in the distribution of mtDNA in cells. PMID: 26445116
  26. Report failed upregulation of TFAM protein and mitochondrial DNA in oxidatively deficient fibers in skeletal muscle of chronic obstructive pulmonary disease patients. PMID: 26893822
  27. our results revealed that stimulation of mitochondrial biogenesis by carvedilol resulted in functional gain of the mitochondria by showing increased oxygen consumption and mitochondrial respiratory rate. PMID: 26797282
  28. Data show that mtTFA proteins are highly expressed in cancer and drug-resistant cells compared to normal cells, and the mtTFA expression is upregulated by signals of oxidative and DNA damage stress in cancer cells. [review] PMID: 26307971
  29. TFAM overexpression suppressed mitoROS and their upregulation in rat cardiomyocytes. PMID: 25822152
  30. TFAM dimerization enhances mitochondrial DNA compaction by promoting looping of the DNA. PMID: 24435062
  31. The combination of strong mtTFA expression and a high survivin index may predict a poor prognosis in patients with pancreatic ductal adenocarcinoma PMID: 25108120
  32. expression of PGC1alpha and TFAM varies between ovarian carcinoma subtypes; clear-cell carcinoma consists of undetectability of PGC1alpha/TFAM, and low ERalpha/Ki-67. high-grade serous carcinomas had a converse state of PGC1alpha/TFAM, ERalpha positivity and high Ki-67 index PMID: 25243473
  33. Methylation of TFAM promoters changed 2 days after gastric bypass. PMID: 24837562
  34. These data disclose a novel mechanism by which ERK1/2 regulates mitochondrial function through direct phosphorylation of TFAM. PMID: 24768991
  35. indicating that miRNA-214 and MTFA may become important candidates for developing promising therapeutic strategies for the treatment of cervical cancer PMID: 25556274
  36. Data suggest that miitochondrial transcription factor A (mtTFA) may be a novel target for the treatment of pancreatic ductal adenocarcinoma (PDAC). PMID: 24622070
  37. The results suggest that the expression of mtTFA mRNA and protein is down-regulated in the lung tissue from the COPD patients with squamous cell lung cancer, and the level of mtTFA protein is related to apoptosis of pulmonary vascular endothelial cells. PMID: 24367550
  38. The mitochondria targeting sequence-deficient hTFAM also repressed Tfam promoter activity to the same degree as hTFAM. PMID: 24875355
  39. These data show that the TFAM SNP rs2306604 A allele may be a risk factor for Parkinson's disease dementia, particularly in males, but not for dementia with Lewy bodies PMID: 24184878
  40. The results reveal the organization of TFAM, POLRMT and TFB2M around the light-strand promoter and represent the first structural characterization of the entire mitochondrial transcriptional initiation complex. PMID: 24413562
  41. results demonstrate that TFAM uniformly coats the whole mitochondrial genome, with no evidence of robust TFAM binding to the nuclear genome PMID: 23991223
  42. Taken together, our data suggested that TFAM plays a crucial role in regulating mtDNA amplification and mitochondrial biogenesis under IR conditions. PMID: 23645454
  43. overexpression of miRNA-23b in U251 cells markedly inhibited the proliferation, cell cycle progression, migration and colony formation, while overexpression of TFAM significantly enhanced these biological processes PMID: 24002170
  44. In cells with normal mitochondrial DNA levels, phosphorylated TFAM is degraded by Lon. PMID: 23201127
  45. TFAM protein sliding and DNA denaturation are essential for mitochondrial DNA organization. PMID: 22910359
  46. Findings suggest that transcription factor A (TFAM) is absolutely required to recruit the transcription machinery during initiation of transcription. PMID: 23012404
  47. The expression of TFAM protein was not significantly reduced in ClpX-knockdown cells. PMID: 22841477
  48. Recombinant TFAM increased mitochondrial DNA and abolished the activation of nuclear factor of activated T cells (NFAT), which is well known to activate pathological hypertrophy. PMID: 22709542
  49. study demonstrates that mtDNA damage is involved in liver damage in extrahepatic cholestatic patients. The mtDNA damage is attributable to the loss of TFAM PMID: 22306509
  50. Plasmacytoid dendritic cells (pDC) contribute to sterile immune responses by recognizing the mitochondrial component of necrotic cells (TFAM), with TFAM and mitochondrial DNA as likely mediators of pDC activation. PMID: 22675199

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Involvement in disease Mitochondrial DNA depletion syndrome 15, hepatocerebral type (MTDPS15)
Subcellular Location Mitochondrion, Mitochondrion matrix, mitochondrion nucleoid
Database Links

HGNC: 11741

OMIM: 600438

KEGG: hsa:7019

STRING: 9606.ENSP00000420588

UniGene: Hs.594250

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