TMEM14A Antibody

Datasheet

Code	CSB-PA023720LA01HU
Size	US$166
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Image	IHC image of CSB-PA023720LA01HU diluted at 1:350 and staining in paraffin-embedded human pancreatic cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA023720LA01HU diluted at 1:350 and staining in paraffin-embedded human ovarian cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Western blot All lanes: TMEM14A antibody at 0.8µg/ml + PC-3 whole cell lysate Secondary Goat polyclonal to rabbit IgG at 1/10000 dilution Predicted band size: 11 kDa Observed band size: 11 kDa Immunofluorescent analysis of PC-3 cells using CSB-PA023720LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TMEM14A Polyclonal antibody

Uniprot No.

Q9Y6G1

Target Names

TMEM14A

Alternative Names

TMEM14A; C6orf73; PTD011; Transmembrane protein 14A

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Transmembrane protein 14A protein (45-78AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TMEM14A Antibody (Product code: CSB-PA023720LA01HU) is Non-conjugated. For TMEM14A Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA023720LB01HU	TMEM14A Antibody, HRP conjugated	ELISA
FITC	CSB-PA023720LC01HU	TMEM14A Antibody, FITC conjugated
Biotin	CSB-PA023720LD01HU	TMEM14A Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Inhibits apoptosis via negative regulation of the mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway.

Gene References into Functions

these results indicate that TMEM14A has a pro-tumorigenic effect in ovarian cancer cells, suggesting an important role of this protein in ovarian cancer oncogenesis and metastasis. PMID: 26896463
findings show for the first time that TMEM14A inhibits apoptosis by blocking the mitochondrial permeability transition and stabilizing mitochondrial membrane potential PMID: 21723035

Subcellular Location

Mitochondrion membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane.

Protein Families

TMEM14 family

Tissue Specificity

Expressed at significantly higher levels in ovarian cancer tissues than in normal tissues (at protein level).

Database Links

HGNC: 21076

KEGG: hsa:28978

UniGene: Hs.94896

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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