TMEM176B Antibody

Datasheet

Citations (1)

Code	CSB-PA023758LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: THP-1 whole cell lysate(30µg), SY5Y whole cell lysate(30µg), Hela whole cell lysate(30µg), K562 whole cell lysate(30µg) All lanes: TMEM176B antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/20000 dilution Predicted band size: 30,26 kDa Observed band size: 30 kDa Exposure time：120s Immunofluorescence staining of Hela cell with CSB-PA023758LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and and permeated by 0.2% TritonX-100 for 15 min. Then 10% normal goat serum to block non-specific protein-protein interactions . The cells were then incubated with the antibody overnight at 4℃. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Immunofluorescence staining of Hela cell with 5% goat serum, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Product Details
Citations
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Customer Reviews and Q&A
Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TMEM176B Polyclonal antibody

Uniprot No.

Q3YBM2

Target Names

TMEM176B

Alternative Names

TMEM176B; LR8; Transmembrane protein 176B; Protein LR8

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Synthesized peptide derived from human TMEM176B

Immunogen Species

Homo sapiens (Human)

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen affinity purification

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:10-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

To produce the TMEM176B antibody, a rabbit was first immunized with the recombinant human transmembrane protein 176B (1-64aa). Following this, the rabbit serum was collected and the antibody was isolated and purified through protein G affinity chromatography.

The resulting antibody showed high purity levels of up to 95% and displayed reactivity solely with human TMEM176B protein. Quality verification tests were conducted on the rabbit anti-human TMEM176B polyclonal antibody, including ELISA, WB, IHC, and IF, confirming its suitability for use in various applications.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Citations

Single-cell mRNA-regulation analysis reveals cell type-specific mechanisms of type 2 diabetes J Martinez-Lopez,bioRxiv,2023

Customer Reviews and Q&A

■ Customer Reviews

Average Rating:

5.0 - 1 reviews

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Applications : Immunohistochemistry

Sample type: tissues

Review: TMEM176B protein is expressed in human beta cells.

By Anonymous

Target Background

Function

May play a role in the process of maturation of dendritic cells. Required for the development of cerebellar granule cells.

Gene References into Functions

Study characterized Tmem176a and b expression in RORgamma t+ lymphocytes at transcriptional and protein levels and present evidence that both genes exert a redundant ion channel function related to a colocalisation in close proximity to the Golgi apparatus. PMID: 27009467
human TMEM176A and 176B protein levels are significantly elevated in lymphoma, but not in normal tissues PMID: 22244448

Subcellular Location

Nucleus membrane; Multi-pass membrane protein.

Protein Families

TMEM176 family

Tissue Specificity

Expressed in lung and dermal fibroblasts.

Database Links

HGNC: 29596

OMIM: 610385

KEGG: hsa:28959

STRING: 9606.ENSP00000318409

UniGene: Hs.647090

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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