TMEM214 Antibody

Code CSB-PA023808LA01HU
Size US$166
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  • Western Blot
    Positive WB detected in: K562 whole cell lysate, HepG2 whole cell lysate, HL-60 whole cell lysate, A549 whole cell lysate, U251 whole cell lysate
    All lanes: TMEM214 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 78, 73 kDa
    Observed band size: 78 kDa

  • Immunoprecipitating TMEM214 in HepG2 whole cell lysate
    Lane 1: Rabbit control IgG instead of CSB-PA023808LA01HU in HepG2 whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/50000)
    Lane 2: CSB-PA023808LA01HU (5ug) + HepG2 whole cell lysate (0.5mg)
    Lane 3: HepG2 whole cell lysate (20ug)

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) TMEM214 Polyclonal antibody
Uniprot No.
Target Names
TMEM214
Alternative Names
TMEM214; PP446; Transmembrane protein 214
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human Transmembrane protein 214 protein (2-236AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The TMEM214 Antibody (Product code: CSB-PA023808LA01HU) is Non-conjugated. For TMEM214 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA023808LB01HU TMEM214 Antibody, HRP conjugated ELISA
FITC CSB-PA023808LC01HU TMEM214 Antibody, FITC conjugated
Biotin CSB-PA023808LD01HU TMEM214 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IP
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IP 1:200-1:2000
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Critical mediator, in cooperation with CASP4, of endoplasmic reticulum-stress induced apoptosis. Required or the activation of CASP4 following endoplasmic reticulum stress.
Gene References into Functions
  1. TMEM214 is essential for endoplasmic reticulum (ER) stress-induced apoptosis by acting as an anchor for recruitment of procaspase 4 to the ER and its subsequent activation. PMID: 23661706
Subcellular Location
Endoplasmic reticulum membrane; Multi-pass membrane protein.
Protein Families
TMEM214 family
Database Links

HGNC: 25983

OMIM: 615301

KEGG: hsa:54867

STRING: 9606.ENSP00000238788

UniGene: Hs.533934

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