TRAF7 Antibody

Datasheet

Code	CSB-PA757671LA01HU
Size	US$166
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Image	IHC image of CSB-PA757671LA01HU diluted at 1:200 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA757671LA01HU at 1:66, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TRAF7 Polyclonal antibody

Uniprot No.

Q6Q0C0

Target Names

TRAF7

Alternative Names

E3 ubiquitin protein ligase TRAF7 antibody; E3 ubiquitin-protein ligase TRAF7 antibody; RFWD1 antibody; Ring finger and WD repeat domain 1 antibody; RING finger and WD repeat-containing protein 1 antibody; RING finger protein 119 antibody; RNF119 antibody; TNF receptor associated factor 7 antibody; TNF receptor-associated factor 7 antibody; TRAF7 antibody; TRAF7_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human E3 ubiquitin-protein ligase TRAF7 protein (1-90AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TRAF7 Antibody (Product code: CSB-PA757671LA01HU) is Non-conjugated. For TRAF7 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA757671LB01HU	TRAF7 Antibody, HRP conjugated	ELISA
FITC	CSB-PA757671LC01HU	TRAF7 Antibody, FITC conjugated
Biotin	CSB-PA757671LD01HU	TRAF7 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC, IF

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500
IF	1:50-1:200

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

E3 ubiquitin ligase capable of auto-ubiquitination, following phosphorylation by MAP3K3. Potentiates MAP3K3-mediated activation of the NF-kappa-B, JUN/AP1 and DDIT3 transcriptional regulators. Induces apoptosis when overexpressed. Plays a role in the phosphorylation of MAPK1 and/or MAPK3, probably via its interaction with MAP3K3.

Gene References into Functions

TRAF7 mutations icause intraneural perineuriomas. PMID: 28019650
TRAF7 is a direct target of miR-126 in human umbilical cord vascular endothelial cells. PMID: 25318608
Downregulation of ubiquitin E3 ligase TNF receptor-associated factor 7 leads to stabilization of p53 in breast cancer. PMID: 23128672
The findings of this study suggested an essential contribution of combined KLF4 K409Q and TRAF7 mutations in the genesis of secretory meningioma and demonstrate a role for TRAF7 alterations in other non-NF2 meningiomas. PMID: 23404370
Nearly one-fourth of all meningiomas have mutations in TRAF7. PMID: 23348505
TRAF7 is involved in signal transduction pathways that lead either to activation or repression of NF-kappaB transcription factor. PMID: 22105767
an important role for TRAF7 in the activation of JNK following TNFalpha stimulation and involvement of this protein in regulating the turnover of c-FLIP PMID: 22219201
this study identifies TRAF7 as a NEMO- and p65-interacting molecule and brings important information on the ubiquitination events that control NF-kappaB transcriptional activity. PMID: 21518757
TRAF7 potentiates MEKK3-induced AP1 and CHOP activation and induces apoptosis PMID: 15001576

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Subcellular Location

Cytoplasmic vesicle. Note=Colocalizes with MAP3K3 to vesicle-like structures throughout the cytoplasm.

Protein Families

WD repeat TRAF7 family

Tissue Specificity

Ubiquitously expressed with high levels in skeletal muscle, heart, colon, spleen, kidney, liver and placenta.

Database Links

HGNC: 20456

OMIM: 606692

KEGG: hsa:84231

STRING: 9606.ENSP00000318944

UniGene: Hs.713632

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Protein Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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