Name: TRIB1 Antibody
Brand: CUSABIO
SKU: CSB-PA836296LA01HU

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TRIB1 Polyclonal antibody

Uniprot No.

Q96RU8

Target Names

TRIB1

Alternative Names

C8FW antibody; G-protein-coupled receptor-induced gene 2 protein antibody; GIG-2 antibody; GIG2 antibody; SKIP1 antibody; TRB-1 antibody; TRB1 antibody; Trib1 antibody; TRIB1_HUMAN antibody; Tribbles homolog 1 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Tribbles homolog 1 protein (3-91AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TRIB1 Antibody (Product code: CSB-PA836296LA01HU) is Non-conjugated. For TRIB1 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA836296LB01HU	TRIB1 Antibody, HRP conjugated	ELISA
FITC	CSB-PA836296LC01HU	TRIB1 Antibody, FITC conjugated
Biotin	CSB-PA836296LD01HU	TRIB1 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Adapter protein involved in protein degradation by interacting with COP1 ubiquitin ligase. The COP1-binding motif is masked by autoinhibitory interactions with the protein kinase domain. Serves to alter COP1 substrate specificity by directing the activity of COP1 toward CEBPA. Binds selectively the recognition sequence of CEBPA. Regulates myeloid cell differentiation by altering the expression of CEBPA in a COP1-dependent manner. Controls macrophage, eosinophil and neutrophil differentiation via the COP1-binding domain. Interacts with MAPK kinases and regulates activation of MAP kinases, but has no kinase activity.

Gene References into Functions

findings support the hypothesis that TRIB1 gene expression in human umbilical vein endothelial cells depends on the duration of intrauterine exposure to hyperglycaemia PMID: 29502537
our study reveals that TRIB1 promotes CRC cell migration and invasion by up-regulating the expression of MMP-2 via the activation of FAK/Src and ERK pathways, knockdown of TRIB1 expression in CRC cells abolishes these effects. PMID: 28624785
role of TRIB1 in cell cycle and survival that is mediated via the modulation of NFkappaB signaling. PMID: 28087598
the co-operativity observed between MYC and TRIB1 in the absence of PML/RARA show that, outside of acute promyelocytic leukemia, gain of both genes may drive selection for trisomy 8. PMID: 27390356
The crucial role of TRIB1 in cisplatin-induced enrichment of CSC and drug resistance was verified by knockdown TRIB1. Interestingly, cisplatin treatment also contributed to the increasement of HDAC, the interaction of TRIB1 with HDAC, and inactivation of p53. PMID: 28406482
Trib1 formed a complex with pHDAC1. PMID: 26521947
Studies indicate that tribbles homolog 1 (Drosophila) protein appear to be involved in some of the most common diseases, such as cancer, metabolic disease and hyperlipidaemia. PMID: 26517921
Studies suggest that pseudo-kinase family of tribbles (TRIB) proteins TRIB1, TRIB2 and TRIB3 play roles in pathogenesis of rheumatoid arthritis (RA) and osteoarthritis. PMID: 26517922
Studies indicate that the minor allele of a single nucleotide polymorphism (SNP, rs6982502) in the regulatory sequence reduces the activity of the tribbles homolog 1 (Drosophila) protein (TRIB1) promoter. PMID: 26517924
Studies suggest that pseudo-kinase family of tribbles (TRIB) proteins TRIB1, TRIB2 and TRIB3 were involved in the pathogenesis of inflammation. PMID: 26517925
Studies indicate that tribbles homolog 1 (Drosophila) protein (TRIB1) interacts with the master molecule of Tregs, forkhead box P3 (FOXP3), a transcription factor essential for Treg suppressive activity. PMID: 26517926
Studies indicate that tribbles homolog 1 (Drosophila) protein (tribbles-1; TRIB1) is an important modulator of human energy metabolism and metabolic syndromes. PMID: 26517927
Studies indicate that small molecules can reveal rate-limiting signalling outputs and functions of pseudo-kinase family of tribbles (TRIB) proteins TRIB1, TRIB2 and TRIB3 in cells and intact organisms, serving as guides for the development of new drugs. PMID: 26517930
Studies indicate that overexpression of the wild-type tribbles homolog 1 (Drosophila) protein (Trib1) gene effectively induces leukaemia. PMID: 26517931
Studies show that TRIB1 and TRIB2 are highly expressed in molecularly-defined sub-types of acute myeloid leukemia (AML). PMID: 26517933
Studies show a remarkable reduction in tribbles 2 protein (Trib2) expression during oocyte maturation whereas tribbles 1 protein (Trib1) and tribbles 3 protein (Trib3) expression was significantly increased during this process. PMID: 26517934
SNP rs17321515 associated with plasma triglycerides level and increasing risk of coronary heart disease in male Chinese Han population PMID: 25986010
These studies indicated that SAP18 expression enhanced the recruitment of mSin3A in coordination with TRIB1 to MTTP regulatory elements and increased MTTP expression. PMID: 25921304
TRB1 negatively regulates tumor-suppressor activity of p53 through p53 deacetylation PMID: 25832642
trait-specific genetic risk scores are robustly associated with 10-yr changes in lipid levels and three individual SNPs in APOE, TRIB1, and APOA1 were strongly associated with 10-yr changes in lipid levels PMID: 24922540
our study indicated that the TRIB1 gene, especially the rs2980879 polymorphism, is related to the development of pancreatic cancer in the Chinese Han population. PMID: 25117373
genetic association studies in Japan: Data suggest SNPs in TRIB1 (rs2954021), GCKR (glucokinase regulatory protein, rs780094), and PNPLA3 (patatin-like phospholipase domain containing 3, rs738409) are associated with nonalcoholic fatty liver disease. PMID: 24785259
TRIB1 was related to endoplasmic reticulum (ER) pathways. PMID: 24962028
association with plasma triglyceride concentrations PMID: 24895164
The TRIB1 rs2954029 is associated with serum triglyceride concentrations in Japanese community-dwelling women. PMID: 24910200
These data suggested that the modulation of TRIB1 expression affects hepatic lipogenesis and glycogenesis through multiple molecular interactions. PMID: 24389359
High TRIB1 expression is associated with prostate cancer. PMID: 24382668
Trib1 acts as an activator of the MAPK pathway. PMID: 24161842
We discuss the role of Tribs as central signaling mediators in different subtypes of acute leukemia and propose that inhibition of dysregulated Trib signaling may be therapeutically beneficial. PMID: 23550039
tribbles-1 is a novel binding partner of Foxp3 in regulatory T cells PMID: 23417677
Allelic variants of TRIB1 are independently involved in regulation of lipid metabolism and sleep. PMID: 22832862
Studies indicate that three loci for lipid levels identified by GWAS has identified functional genes GALNT2, TRIB1, and SORT1, and a functional variant at SORT1. PMID: 22418572
Studies indicate tribbles 1 and tribbles 3 as regulators of lipid level and disease susceptibility genes in metabolic syndrome and type 2 diabetes. PMID: 22274752
TRIB1 R107L gain-of-function mutation is associated with acute megakaryocytic leukemia. PMID: 22294728
The associations of TRIB1 rs17321515 SNP and serum lipid levels are different between the Mulao and Han populations. PMID: 22145581
genetic association studies in Spain: A TRIB1 SNP (rs17321515) is associated with familial hypercholesterolemia. Smoking status and presence of arcus cornealis modulate effect of this TRIB1 SNP on plasma lipid levels in such patients. PMID: 20692138
Data show no differences in triglyceride or total cholesterol levels in relation to any allelic variants of ANGPTL3, CILP2, or TRIB1 SNPs. PMID: 21691831
TRIB1 polymorphisms were associated with increased risk of ischemic heart disease and myocardial infarction. PMID: 21071687
Tribbles homolog 1 (Trib1) functions as a negative regulator of retinoic acid receptors. PMID: 21053424
Trib1 may be a key mediator between the RTK-MAPK pathway and the C/EBP transcription factor in myeloid leukemogenesis. PMID: 20610816
low trb-1 expression was associated with inactive C/EBPalpha PMID: 20005259
Data show that SNPs associated with TG in normolipidemic samples, including APOA5, TRIB1, TBL2, GCKR, GALNT2 and ANGPTL3 were significantly associated with HLP types 2B, 3, 4 and 5. PMID: 19656773
Tribbles controls both the extent and the specificity of MAPK kinase activation of MAPK PMID: 15299019
used chemical shift perturbation experiments to show the possible SKIP binding interface on PPIL1 PMID: 16595688
trb-1 has an important role in vascular smooth muscle cell biology PMID: 17452330

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Protein Families

Protein kinase superfamily, CAMK Ser/Thr protein kinase family, Tribbles subfamily

Tissue Specificity

Expressed in most human tissues with the highest levels in skeletal muscle, thyroid gland, pancreas, peripheral blood leukocytes, and bone marrow.

Database Links

HGNC: 16891

OMIM: 609461

KEGG: hsa:10221

STRING: 9606.ENSP00000312150

UniGene: Hs.444947

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Code	CSB-PA836296LA01HU
Size	US$166
	Order now
Image	Western Blot Positive WB detected in: Raji whole cell lysate All lanes: TRIB1 antibody at 4.3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 42, 24 kDa Observed band size: 42 kDa IHC image of CSB-PA836296LA01HU diluted at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA836296LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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TRIB1 Antibody

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Target Background