TRIB1 Antibody

Code CSB-PA836296LA01HU
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Image
  • Western Blot
    Positive WB detected in: Raji whole cell lysate
    All lanes: TRIB1 antibody at 4.3µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 42, 24 kDa
    Observed band size: 42 kDa

  • IHC image of CSB-PA836296LA01HU diluted at 1:500 and staining in paraffin-embedded human skeletal muscle tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA836296LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Product Details

Full Product Name Rabbit anti-Homo sapiens (Human) TRIB1 Polyclonal antibody
Uniprot No. Q96RU8
Target Names TRIB1
Alternative Names C8FW antibody; G-protein-coupled receptor-induced gene 2 protein antibody; GIG-2 antibody; GIG2 antibody; SKIP1 antibody; TRB-1 antibody; TRB1 antibody; Trib1 antibody; TRIB1_HUMAN antibody; Tribbles homolog 1 antibody
Raised in Rabbit
Species Reactivity Human
Immunogen Recombinant Human Tribbles homolog 1 protein (3-91AA)
Immunogen Species Homo sapiens (Human)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method >95%, Protein G purified
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:5000
IHC 1:500-1:1000
IF 1:50-1:200
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Data

Function Adapter protein involved in protein degradation by interacting with RFWD2/COP1 ubiquitin ligase
Gene References into Functions
  1. findings support the hypothesis that TRIB1 gene expression in human umbilical vein endothelial cells depends on the duration of intrauterine exposure to hyperglycaemia PMID: 29502537
  2. our study reveals that TRIB1 promotes CRC cell migration and invasion by up-regulating the expression of MMP-2 via the activation of FAK/Src and ERK pathways, knockdown of TRIB1 expression in CRC cells abolishes these effects. PMID: 28624785
  3. role of TRIB1 in cell cycle and survival that is mediated via the modulation of NFkappaB signaling. PMID: 28087598
  4. the co-operativity observed between MYC and TRIB1 in the absence of PML/RARA show that, outside of acute promyelocytic leukemia, gain of both genes may drive selection for trisomy 8. PMID: 27390356
  5. The crucial role of TRIB1 in cisplatin-induced enrichment of CSC and drug resistance was verified by knockdown TRIB1. Interestingly, cisplatin treatment also contributed to the increasement of HDAC, the interaction of TRIB1 with HDAC, and inactivation of p53. PMID: 28406482
  6. Trib1 formed a complex with pHDAC1. PMID: 26521947
  7. Studies indicate that tribbles homolog 1 (Drosophila) protein appear to be involved in some of the most common diseases, such as cancer, metabolic disease and hyperlipidaemia. PMID: 26517921
  8. Studies suggest that pseudo-kinase family of tribbles (TRIB) proteins TRIB1, TRIB2 and TRIB3 play roles in pathogenesis of rheumatoid arthritis (RA) and osteoarthritis. PMID: 26517922
  9. Studies indicate that the minor allele of a single nucleotide polymorphism (SNP, rs6982502) in the regulatory sequence reduces the activity of the tribbles homolog 1 (Drosophila) protein (TRIB1) promoter. PMID: 26517924
  10. Studies suggest that pseudo-kinase family of tribbles (TRIB) proteins TRIB1, TRIB2 and TRIB3 were involved in the pathogenesis of inflammation. PMID: 26517925
  11. Studies indicate that tribbles homolog 1 (Drosophila) protein (TRIB1) interacts with the master molecule of Tregs, forkhead box P3 (FOXP3), a transcription factor essential for Treg suppressive activity. PMID: 26517926
  12. Studies indicate that tribbles homolog 1 (Drosophila) protein (tribbles-1; TRIB1) is an important modulator of human energy metabolism and metabolic syndromes. PMID: 26517927
  13. Studies indicate that small molecules can reveal rate-limiting signalling outputs and functions of pseudo-kinase family of tribbles (TRIB) proteins TRIB1, TRIB2 and TRIB3 in cells and intact organisms, serving as guides for the development of new drugs. PMID: 26517930
  14. Studies indicate that overexpression of the wild-type tribbles homolog 1 (Drosophila) protein (Trib1) gene effectively induces leukaemia. PMID: 26517931
  15. Studies show that TRIB1 and TRIB2 are highly expressed in molecularly-defined sub-types of acute myeloid leukemia (AML). PMID: 26517933
  16. Studies show a remarkable reduction in tribbles 2 protein (Trib2) expression during oocyte maturation whereas tribbles 1 protein (Trib1) and tribbles 3 protein (Trib3) expression was significantly increased during this process. PMID: 26517934
  17. SNP rs17321515 associated with plasma triglycerides level and increasing risk of coronary heart disease in male Chinese Han population PMID: 25986010
  18. These studies indicated that SAP18 expression enhanced the recruitment of mSin3A in coordination with TRIB1 to MTTP regulatory elements and increased MTTP expression. PMID: 25921304
  19. TRB1 negatively regulates tumor-suppressor activity of p53 through p53 deacetylation PMID: 25832642
  20. trait-specific genetic risk scores are robustly associated with 10-yr changes in lipid levels and three individual SNPs in APOE, TRIB1, and APOA1 were strongly associated with 10-yr changes in lipid levels PMID: 24922540
  21. our study indicated that the TRIB1 gene, especially the rs2980879 polymorphism, is related to the development of pancreatic cancer in the Chinese Han population. PMID: 25117373
  22. genetic association studies in Japan: Data suggest SNPs in TRIB1 (rs2954021), GCKR (glucokinase regulatory protein, rs780094), and PNPLA3 (patatin-like phospholipase domain containing 3, rs738409) are associated with nonalcoholic fatty liver disease. PMID: 24785259
  23. TRIB1 was related to endoplasmic reticulum (ER) pathways. PMID: 24962028
  24. association with plasma triglyceride concentrations PMID: 24895164
  25. The TRIB1 rs2954029 is associated with serum triglyceride concentrations in Japanese community-dwelling women. PMID: 24910200
  26. These data suggested that the modulation of TRIB1 expression affects hepatic lipogenesis and glycogenesis through multiple molecular interactions. PMID: 24389359
  27. High TRIB1 expression is associated with prostate cancer. PMID: 24382668
  28. Trib1 acts as an activator of the MAPK pathway. PMID: 24161842
  29. We discuss the role of Tribs as central signaling mediators in different subtypes of acute leukemia and propose that inhibition of dysregulated Trib signaling may be therapeutically beneficial. PMID: 23550039
  30. tribbles-1 is a novel binding partner of Foxp3 in regulatory T cells PMID: 23417677
  31. Allelic variants of TRIB1 are independently involved in regulation of lipid metabolism and sleep. PMID: 22832862
  32. Studies indicate that three loci for lipid levels identified by GWAS has identified functional genes GALNT2, TRIB1, and SORT1, and a functional variant at SORT1. PMID: 22418572
  33. Studies indicate tribbles 1 and tribbles 3 as regulators of lipid level and disease susceptibility genes in metabolic syndrome and type 2 diabetes. PMID: 22274752
  34. TRIB1 R107L gain-of-function mutation is associated with acute megakaryocytic leukemia. PMID: 22294728
  35. The associations of TRIB1 rs17321515 SNP and serum lipid levels are different between the Mulao and Han populations. PMID: 22145581
  36. genetic association studies in Spain: A TRIB1 SNP (rs17321515) is associated with familial hypercholesterolemia. Smoking status and presence of arcus cornealis modulate effect of this TRIB1 SNP on plasma lipid levels in such patients. PMID: 20692138
  37. Data show no differences in triglyceride or total cholesterol levels in relation to any allelic variants of ANGPTL3, CILP2, or TRIB1 SNPs. PMID: 21691831
  38. TRIB1 polymorphisms were associated with increased risk of ischemic heart disease and myocardial infarction. PMID: 21071687
  39. Tribbles homolog 1 (Trib1) functions as a negative regulator of retinoic acid receptors. PMID: 21053424
  40. Trib1 may be a key mediator between the RTK-MAPK pathway and the C/EBP transcription factor in myeloid leukemogenesis. PMID: 20610816
  41. low trb-1 expression was associated with inactive C/EBPalpha PMID: 20005259
  42. Data show that SNPs associated with TG in normolipidemic samples, including APOA5, TRIB1, TBL2, GCKR, GALNT2 and ANGPTL3 were significantly associated with HLP types 2B, 3, 4 and 5. PMID: 19656773
  43. Tribbles controls both the extent and the specificity of MAPK kinase activation of MAPK PMID: 15299019
  44. used chemical shift perturbation experiments to show the possible SKIP binding interface on PPIL1 PMID: 16595688
  45. trb-1 has an important role in vascular smooth muscle cell biology PMID: 17452330

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Protein Families Protein kinase superfamily, CAMK Ser/Thr protein kinase family, Tribbles subfamily
Tissue Specificity Expressed in most human tissues with the highest levels in skeletal muscle, thyroid gland, pancreas, peripheral blood leukocytes, and bone marrow.
Database Links

HGNC: 16891

OMIM: 609461

KEGG: hsa:10221

STRING: 9606.ENSP00000312150

UniGene: Hs.444947

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