TSPAN15 Antibody

Datasheet

Code	CSB-PA025152LA01HU
Size	US$166
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Image	Immunofluorescence staining of A549 cell with CSB-PA025152LA01HU at 1:30, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western Blot Positive WB detected in: MCF7 whole cell lysate, Hela whole cell lysate, A549 whole cell lysate, JK whole cell lysate, 293 whole cell lysate All lanes: TSPAN15 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 34 kDa Observed band size: 34 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) TSPAN15 Polyclonal antibody

Uniprot No.

O95858

Target Names

TSPAN15

Alternative Names

TSPAN15; NET7; TM4SF15; UNQ677/PRO1311; Tetraspanin-15; Tspan-15; Tetraspan NET-7; Transmembrane 4 superfamily member 15

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Tetraspanin-15 protein (115-235AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The TSPAN15 Antibody (Product code: CSB-PA025152LA01HU) is Non-conjugated. For TSPAN15 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA025152LB01HU	TSPAN15 Antibody, HRP conjugated	ELISA
FITC	CSB-PA025152LC01HU	TSPAN15 Antibody, FITC conjugated
Biotin	CSB-PA025152LD01HU	TSPAN15 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function

Regulates maturation and trafficking of the transmembrane metalloprotease ADAM10. Promotes ADAM10-mediated cleavage of CDH2. Negatively regulates ligand-induced Notch activity probably by regulating ADAM10 activity.

Gene References into Functions

TSPAN15 interacts with BTRC to promote oesophageal squamous cell carcinoma metastasis via activating NF-kappaB signaling. PMID: 29650964
Meta-analysis of 65,734 individuals identifies TSPAN15 and SLC44A2 as two susceptibility loci for venous thromboembolism. PMID: 25772935

Subcellular Location

Cell membrane; Multi-pass membrane protein. Late endosome membrane.

Protein Families

Tetraspanin (TM4SF) family

Database Links

HGNC: 23298

OMIM: 613140

KEGG: hsa:23555

UniGene: Hs.499941

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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