Product Details

Uniprot No.

Target Names

TUT1

Alternative Names

alpha regulated poly(A) polymerase antibody; nuclear speckle targeted phosphatidylinositol 4 phosphate 5 kinase type I antibody; nuclear speckle targeted PIPK1A regulated poly(A) polymerase antibody; PAP associated domain containing 2 antibody; PAPD2 antibody; poly(A) polymerase associated domain containing 2 antibody; RBM21 antibody; RNA binding motif protein 21 antibody; RNA-binding motif protein 21 antibody; RNA-binding protein 21 antibody; Speckle targeted PIP5K1A-regulated poly(A) polymerase antibody; Star-PAP antibody; STARPAP antibody; STPAP_HUMAN antibody; terminal uridylyl transferase 1; U6 snRNA specific antibody; TUT1 antibody; TUTase antibody; U6 snRNA specific terminal uridylyltransferase 1 antibody; U6 snRNA-specific terminal uridylyltransferase 1 antibody; U6 TUTase antibody; U6-TUTase antibody; URLC6 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Synthesized peptide derived from the Internal region of Human TUTase.

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

WB, IHC, ELISA

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:100-1:300
ELISA	1:20000

Protocols

Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Poly(A) polymerase that creates the 3'-poly(A) tail of specific pre-mRNAs. Localizes to nuclear speckles together with PIP5K1A and mediates polyadenylation of a select set of mRNAs, such as HMOX1. In addition to polyadenylation, it is also required for the 3'-end cleavage of pre-mRNAs: binds to the 3'UTR of targeted pre-mRNAs and promotes the recruitment and assembly of the CPSF complex on the 3'UTR of pre-mRNAs. In addition to adenylyltransferase activity, also has uridylyltransferase activity. However, the ATP ratio is higher than UTP in cells, suggesting that it functions primarily as a poly(A) polymerase. Acts as a specific terminal uridylyltransferase for U6 snRNA in vitro: responsible for a controlled elongation reaction that results in the restoration of the four 3'-terminal UMP-residues found in newly transcribed U6 snRNA. Not involved in replication-dependent histone mRNA degradation.

Gene References into Functions

Crystallographic and biochemical studies of TUT1 revealed the molecular mechanism underlying the specific oligo-uridylylation of the 3'-end of U6 snRNA by TUT1. PMID: 28589955
Star-PAP possesses tumor-suppressing activity. PMID: 28151486
Star-PAP-specific polyadenylation sites usage regulates the expression of the eukaryotic translation initiation factor EIF4A1, the tumor suppressor gene PTEN and the long non-coding RNA NEAT1. PMID: 28911096
Star-PAP recognises a unique nucleotide motif on its target mRNA.CstF-64 and 3'-UTR cis-element determine Star-PAP specificity for target mRNA selection by excluding poly A polymerase. PMID: 26496945
Nucleotidyl transferase TUT1 inhibits lipogenesis in osteosarcoma cells through regulation of microRNA-24 and microRNA-29a. PMID: 25142229
Star-PAP controls E6 mRNA polyadenylation and expression and modulates wild-type p53 levels. PMID: 23416977
The human TUT1 nucleotidyl transferase is a global regulator of microRNA abundance. PMID: 23874977
Star-PAP and its regulatory molecules form a signaling nexus at the 3'-end of target mRNAs to control the expression of select group of genes including the ones involved in stress responses. (Review) PMID: 23306079
PIPKIalpha, PI4,5P(2), and PKCdelta regulate Star-PAP control of BIK expression and induction of apoptosis. PMID: 22244330
The data support a model where Star-PAP binds to the pre-mRNA, recruits the CPSF complex to the 3'-end of pre-mRNA and then defines cleavage by CPSF 73 and subsequent polyadenylation of its target mRNAs. PMID: 21102410
PIPKIalpha co-localizes at nuclear speckles and interacts with a newly identified non-canonical poly(A) polymerase, Star-PAP; the activity of Star-PAP can be specifically regulated by PtdIns4,5P2 PMID: 18288197
These data indicate that CKIalpha, PIPKIalpha, and Star-PAP function together to modulate the production of specific Star-PAP messages. PMID: 18305108

Hide All

Subcellular Location

Nucleus, nucleolus. Nucleus speckle.

Protein Families

DNA polymerase type-B-like family

Tissue Specificity

Widely expressed.

Database Links

HGNC: 26184

OMIM: 610641

KEGG: hsa:64852

STRING: 9606.ENSP00000308000

UniGene: Hs.728983

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Code	CSB-PA050066
Size	US$119
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TUT1 Antibody

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