Product Details

Uniprot No.

Target Names

UBE2T

Alternative Names

Cell proliferation inducing gene 50 protein antibody; Cell proliferation-inducing gene 50 protein antibody; HSPC150 antibody; HSPC150 protein similar to ubiquitin conjugating enzyme antibody; PIG50 antibody; Ube2t antibody; UBE2T_HUMAN antibody; Ubiquitin carrier protein T antibody; Ubiquitin conjugating enzyme antibody; ubiquitin conjugating enzyme E2 T antibody; Ubiquitin conjugating enzyme E2T antibody; Ubiquitin protein ligase T antibody; Ubiquitin-conjugating enzyme E2 T antibody; Ubiquitin-protein ligase T antibody

Raised in

Rabbit

Species Reactivity

Human,Mouse

Immunogen

Synthesized peptide derived from the Internal region of Human UBE2T.

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Isotype

IgG

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Form

Liquid

Tested Applications

WB, ELISA

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
ELISA	1:10000

Protocols

Western Blotting(WB) Protocol
ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Related Products

Target Background

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes monoubiquitination. Involved in mitomycin-C (MMC)-induced DNA repair. Acts as a specific E2 ubiquitin-conjugating enzyme for the Fanconi anemia complex by associating with E3 ubiquitin-protein ligase FANCL and catalyzing monoubiquitination of FANCD2, a key step in the DNA damage pathway. Also mediates monoubiquitination of FANCL and FANCI. May contribute to ubiquitination and degradation of BRCA1. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, but may prefer 'Lys-11'-, 'Lys-27'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination.

Gene References into Functions

Results show that UBE2T expression was increased in gastric tumors. Its suppression altered the expression of epithelial-mesenchymal transition factors and inhibited growth and colony formation, increased G2/M phase cell cycle arrest, and promoted apoptosis in gastric cancer cells. These findings provide evidence that UBE2T plays a critical role in gastric cancer. PMID: 28427240
High UBE2T expression is associated with nasopharyngeal carcinoma. PMID: 26943030
Collectively, our findings suggest UBE2T serves as a promising prognostic factor for HCC and functions as an oncogene. The newly identified miR-543/UBE2T/p53 axis may represent a new potential therapeutic target for HCC intervention. PMID: 28935368
report the identification of a new allosteric pocket on Ube2T through a fragment screening using biophysical methods. Several fragments binding to this site inhibit ubiquitin conjugation in vitro PMID: 28437106
Results showed that UBE2T was overexpressed in osteosarcoma tissues and cell lines. Moreover, UBE2T knockdown inhibited osteosarcoma cell proliferation, migration, and invasion. PMID: 27712593
UBE2T plays an important role in the tumorigenesis of gastric cancer and could act as a potential independent prognostic factor for cancer therapy. PMID: 27020591
Identify critical roles of UBE2T in prostate cancer development and progression. PMID: 26308072
we identified UBE2T as a bona fide FA gene (FANCT) that also may be a rare cancer susceptibility gene. PMID: 26085575
UBE2T is the primary E2 of the FA pathway required for FANCD2 and FANCI activation. PMID: 26119737
Mutations in the gene encoding the E2 conjugating enzyme UBE2T cause Fanconi anemia PMID: 26046368
hypoxic conditions down-regulate UBE2T expression which correlates with an increased sensitivity to crosslinking agents consistent with a defective Fanconi anemia pathway PMID: 21722982
UBE2T is the ubiquitin-conjugating enzyme (E2) in the Fanconi anemia pathway and has a self-inactivation mechanism that could be important for negative regulation of the Fanconi anemia pathway. PMID: 16916645
UBE2T was significantly upregulated in lung cancer tissue and cell lines, suggesting involvement of UBE2T in the malignant cell phenotype. PMID: 18667844
This work therefore establishes a system that provides mechanistic insight into the functions of FANCL and FANCI in the catalysis of FANCD2 monoubiquitination. PMID: 19111657
Upon the occurrence of DNA damage, FANCI becomes monoubiquitinated on Lys-523 by the UBE2T-FANCL pair. PMID: 19589784
Our data imply a critical role of UBE2T in development and/or progression of breast cancer through the interaction with and the regulation of the BRCA1/BARD1 complex. PMID: 19887602

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Involvement in disease

Fanconi anemia complementation group T (FANCT)

Subcellular Location

Nucleus. Note=Accumulates to chromatin.

Protein Families

Ubiquitin-conjugating enzyme family

Database Links

HGNC: 25009

OMIM: 610538

KEGG: hsa:29089

STRING: 9606.ENSP00000356243

UniGene: Hs.5199

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