USP4 Antibody

Datasheet

Code	CSB-PA614393LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate All lanes: USP4 antibody at 6.3µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 109, 104, 36 kDa Observed band size: 109 kDa IHC image of CSB-PA614393LA01HU diluted at 1:500 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. IHC image of CSB-PA614393LA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of U251 cells with CSB-PA614393LA01HU at 1:166, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) USP4 Polyclonal antibody

Uniprot No.

Q13107

Target Names

USP4

Alternative Names

Deubiquitinating enzyme 4 antibody; MGC149848 antibody; MGC149849 antibody; Proto oncogene antibody; Protooncogene antibody; Ubiquitin carboxyl terminal hydrolase 4 antibody; Ubiquitin carboxyl-terminal hydrolase 4 antibody; Ubiquitin specific peptidase 4 (proto oncogene) antibody; Ubiquitin specific peptidase 4 (protooncogene) antibody; Ubiquitin specific peptidase 4 antibody; Ubiquitin specific processing protease 4 antibody; Ubiquitin specific protease 4 (proto oncogene) antibody; Ubiquitin specific protease 4 (protooncogene) antibody; Ubiquitin specific protease 4 antibody; Ubiquitin specific protease proto oncogene antibody; Ubiquitin specific protease protooncogene antibody; Ubiquitin thioesterase 4 antibody; Ubiquitin thiolesterase 4 antibody; Ubiquitin-specific-processing protease 4 antibody; Ubiquitous nuclear protein homolog antibody; UBP4_HUMAN antibody; UNP antibody; UNPH antibody; USP 4 antibody; USP4 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Ubiquitin carboxyl-terminal hydrolase 4 protein (632-810AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The USP4 Antibody (Product code: CSB-PA614393LA01HU) is Non-conjugated. For USP4 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA614393LB01HU	USP4 Antibody, HRP conjugated	ELISA
FITC	CSB-PA614393LC01HU	USP4 Antibody, FITC conjugated
Biotin	CSB-PA614393LD01HU	USP4 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Deubiquitinating enzyme that removes conjugated ubiquitin from target proteins. Deubiquitinates PDPK1. Deubiquitinates TRIM21. Deubiquitinates receptor ADORA2A which increases the amount of functional receptor at the cell surface. May regulate mRNA splicing through deubiquitination of the U4 spliceosomal protein PRPF3. This may prevent its recognition by the U5 component PRPF8 thereby destabilizing interactions within the U4/U6.U5 snRNP. May also play a role in the regulation of quality control in the ER.

Gene References into Functions

Data showed significantly increased expression of USP4 in cancer tissues compared to that in para-carcinoma tissues. PMID: 28946564
The data indicate that USP4 interacts with and deubiquitinates IRF4, and also stabilizes IRF4 protein and promotes IRF4 function to facilitate IL-4 expression in Th2 cells, which may be related to the pathological process of rheumatic heart disease. PMID: 28791349
These results identify USP4 as a novel regulator of Dvl in Wnt/beta-catenin signal and show its involvement in Wnt3a-induced osteoblast differentiation PMID: 27128386
Data suggest that ubiquitin specific protease 4 (USP4) interacts with interferon regulatory factor 8 (IRF8) and, by its Lys48-specific deubiquitinase/endopeptidase activity, stabilizes IRF8 protein levels in regulatory T-lymphocytes; USP4 and IRF8 are also expressed in helper T-lymphocytes. PMID: 28477415
USP4 inhibits p53 and NF-kappaB through deubiquitinating and stabilizing HDAC2 PMID: 26411366
ubiquitin-specific protease 4 (USP4) is a binding partner of RNPS1. PMID: 27990632
Data indicate that programmed cell death 4 (PDCD4) was identified to be a target of ubiquitin-specific protease 4 (USP4), which plays a role as a tumor suppressor. PMID: 27430936
Ubiquitin-specific protease 4 (USP4), recently identified as a beta-catenin-specific deubiquitinylating enzyme, was highly expressed in PC14PE6/LvBr4 cells and involved in the increased stability of beta-catenin protein. PMID: 26883469
USP4 knockdown in HCT116, a colon cancer cell line, reduced invasion and migration activity. PMID: 26189775
USP4 cooperates with CtIP in DNA double-strand break end resection. PMID: 26387952
Results demonstrate that aberrant expression of USP4 contributes to the development and progression of colorectal cancer and reveal a critical mechanism underlying USP4-mediated oncogenic activity. PMID: 26669864
findings thus identify USP4 as a novel DNA repair regulator and invoke a model in which ubiquitin adducts regulate USP enzyme interactions and functions. PMID: 26455393
USP4 and IL-17 mRNA, but not RORgammat mRNA, were significantly elevated in CD4(+) T cells from patients with rheumatic heart disease PMID: 25821221
USP4 overexpression is associated with hepatocellular carcinoma. PMID: 24798342
Authors identified USP4 as a new positive regulator for RIG-I that acts through deubiquitinating K48-linked ubiquitin chains and stabilizing RIG-I. PMID: 23388719
Data suggest that USP4 down-regulates RIP1 (receptor-interacting serine-threonine kinase 1)-mediated TNFalpha (tumor necrosis factor-alpha) activation and promotes TNFalpha-induced apoptosis via deubiquitination of RIP1 in head/neck carcinoma. PMID: 23313255
the interaction with USP4 may regulate the structure and function of the proteasome or the turnover of specific proteasomal substrates. PMID: 23022198
USP4 plays an essential role in negative regulation of the TLR/IL-1R signaling-mediated innate immune response PMID: 22262844
Results uncover USP4 as an important determinant for crosstalk between TGF-beta/TGF-beta type I receptor and AKT signalling pathways. PMID: 22706160
identify the Ubiquitin-Specific Protease 4 (USP4) as an enzyme that removes ubiquitin from PDK1 in vivo and in vitro and co-localizes with PDK1 at the plasma membrane when the two proteins are overexpressed, indicating direct deubiquitination PMID: 22347420
Results demonstrate that USP4 serves as a critical control to downregulate TNFalpha-induced NF-kappaB activation through deubiquitinating TAK1. PMID: 21331078
USP4 deubiquitinates both TRAF2 and TRAF6 in vivo and in vitro in a deubiquitinase activity-dependent manner and inhibits TNFalpha-induced cancer cell migration. PMID: 22029577
Studies indicate that the cylindromatosis/turban tumor syndrome gene (CYLD) ranked highest, followed by acylaminoacyl-peptidase (APEH), dystroglycan (DAG1), macrophage-stimulating protein (MST1) and ubiquitin-specific peptidase 4 (USP4). PMID: 21931648
modulation of USP4 expression may provide a new means to interfere with canonical Wnt signalling in a variety of physiological and pathological conditions PMID: 20141612
These results suggest that UnpEL colocalizes with the unubiquitinated form of Ro52 to the cytoplasmic rod-like structures. PMID: 16316627
Ro52 and UnpEL transregulate each other by ubiquitination and deubiquitination PMID: 16472766

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Subcellular Location

Cytoplasm. Nucleus.

Protein Families

Peptidase C19 family, USP4 subfamily

Tissue Specificity

Overexpressed in small cell tumors and adenocarcinomas of the lung compared to wild-type lung (at protein level). Expressed in the hippocampal neurons.

Database Links

HGNC: 12627

OMIM: 603486

KEGG: hsa:7375

STRING: 9606.ENSP00000265560

UniGene: Hs.403828

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