UST Antibody

Datasheet

Code	CSB-PA897525LA01HU
Size	US$166
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Image	IHC image of CSB-PA897525LA01HU diluted at 1:200 and staining in paraffin-embedded human liver cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) UST Polyclonal antibody

Uniprot No.

Q9Y2C2

Target Names

UST

Alternative Names

2OST antibody; DS2ST antibody; Uronyl 2-sulfotransferase antibody; uronyl-2-sulfotransferase antibody; Ust antibody; UST_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Uronyl 2-sulfotransferase protein (85-183AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The UST Antibody (Product code: CSB-PA897525LA01HU) is Non-conjugated. For UST Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA897525LB01HU	UST Antibody, HRP conjugated	ELISA
FITC	CSB-PA897525LC01HU	UST Antibody, FITC conjugated
Biotin	CSB-PA897525LD01HU	UST Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function

Sulfotransferase that catalyzes the transfer of sulfate to the position 2 of uronyl residues. Has mainly activity toward iduronyl residues in dermatan sulfate, and weaker activity toward glucuronyl residues of chondroitin sulfate. Has no activity toward desulfated N-resulfated heparin.

Gene References into Functions

Results show that overexpression of 2OST alone in the HEK293 cells did not appreciably affect heparan sulfate (HS) chain length. Also, its simultaneous overexpression with Hsepi abolished the effect of Hsepi overexpression on the HS chain lengh. PMID: 27511124
Statistical analysis showed the strongest evidence for a variant located in an intron of UST associated with job-related exhaustion. PMID: 23620144
2OST transfers sulfate preferentially to the GlcA residue located in a unique sequence, -GalNAc(4SO(4))-GlcA-GalNAc(6SO(4))-. PMID: 16192264
analysis of differences and similarities various residues play in the biological roles of the HS-2OST and CS-2OST enzymes PMID: 17227754

Subcellular Location

Golgi apparatus membrane; Single-pass type II membrane protein.

Protein Families

Sulfotransferase 3 family

Tissue Specificity

Widely expressed.

Database Links

HGNC: 17223

OMIM: 610752

KEGG: hsa:10090

STRING: 9606.ENSP00000356433

UniGene: Hs.657370

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