VPS18 Antibody

Datasheet

Code	CSB-PA882173LA01HU
Size	US$166
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Image	IHC image of CSB-PA882173LA01HU diluted at 1:300 and staining in paraffin-embedded human testis tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) VPS18 Polyclonal antibody

Uniprot No.

Q9P253

Target Names

VPS18

Alternative Names

hVPS18 antibody; PEP3 antibody; Vacuolar protein sorting 18 antibody; Vacuolar protein sorting protein 18 antibody; Vacuolar protein sorting-associated protein 18 homolog antibody; vps18 antibody; VPS18_HUMAN antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Vacuolar protein sorting-associated protein 18 homolog protein (298-515AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The VPS18 Antibody (Product code: CSB-PA882173LA01HU) is Non-conjugated. For VPS18 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA882173LB01HU	VPS18 Antibody, HRP conjugated	ELISA
FITC	CSB-PA882173LC01HU	VPS18 Antibody, FITC conjugated
Biotin	CSB-PA882173LD01HU	VPS18 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, IHC

Recommended Dilution

Application	Recommended Dilution
IHC	1:200-1:500

Protocols

ELISA Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Plays a role in vesicle-mediated protein trafficking to lysosomal compartments including the endocytic membrane transport and autophagic pathways. Believed to act as a core component of the putative HOPS and CORVET endosomal tethering complexes which are proposed to be involved in the Rab5-to-Rab7 endosome conversion probably implicating MON1A/B, and via binding SNAREs and SNARE complexes to mediate tethering and docking events during SNARE-mediated membrane fusion. The HOPS complex is proposed to be recruited to Rab7 on the late endosomal membrane and to regulate late endocytic, phagocytic and autophagic traffic towards lysosomes. The CORVET complex is proposed to function as a Rab5 effector to mediate early endosome fusion probably in specific endosome subpopulations. Required for fusion of endosomes and autophagosomes with lysosomes. Involved in dendrite development of Pukinje cells.

Gene References into Functions

VPS18 recruits VPS41 to the human HOPS complex via a RING-RING interaction PMID: 28931724
Depletion of hVps18 or hMon2 reduced the efficient production of infectious HIV-1 virions in human cells. PMID: 21450827
hVPS18 may play an important role in regulation of SNK activity through its ubiquitin ligase PMID: 16203730
ubiquitin binding and ubiquitylation of GGA3-GAT domain are mutually inseparable through a ubiquitin ligase activity of hVPS18 PMID: 16996030

Subcellular Location

Late endosome membrane; Peripheral membrane protein; Cytoplasmic side. Lysosome membrane; Peripheral membrane protein; Cytoplasmic side. Early endosome. Cytoplasmic vesicle, autophagosome. Cytoplasmic vesicle, clathrin-coated vesicle. Note=Cytoplasmic, peripheral membrane protein associated with early endosomes and late endosomes/lysosomes.

Protein Families

VPS18 family

Tissue Specificity

Ubiquitous. Expression was highest in heart and low in lung.

Database Links

HGNC: 15972

OMIM: 608551

KEGG: hsa:57617

STRING: 9606.ENSP00000220509

UniGene: Hs.23876

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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Target Therapy

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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