WDR59 Antibody

Code CSB-PA764810LA01HU
Size US$166
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Image
  • Immunofluorescence staining of U251 cells with CSB-PA764810LA01HU at 1:173, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Western Blot
    Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate
    All lanes: WDR59 antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 110, 65, 63, 47 kDa
    Observed band size: 110 kDa

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) WDR59 Polyclonal antibody
Uniprot No.
Target Names
WDR59
Alternative Names
WDR59 antibody; KIAA1923 antibody; FP977 antibody; GATOR complex protein WDR59 antibody; WD repeat-containing protein 59 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Recombinant Human GATOR complex protein WDR59 protein (619-862AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The WDR59 Antibody (Product code: CSB-PA764810LA01HU) is Non-conjugated. For WDR59 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA764810LB01HU WDR59 Antibody, HRP conjugated ELISA
FITC CSB-PA764810LC01HU WDR59 Antibody, FITC conjugated
Biotin CSB-PA764810LD01HU WDR59 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IF
Recommended Dilution
Application Recommended Dilution
WB 1:1000-1:5000
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Customer Reviews and Q&A

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Target Background

Function
As a component of the GATOR subcomplex GATOR2, functions within the amino acid-sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex. It is negatively regulated by the upstream amino acid sensors SESN2 and CASTOR1.
Subcellular Location
Lysosome membrane.
Protein Families
WD repeat WDR59 family
Database Links

HGNC: 25706

OMIM: 617418

KEGG: hsa:79726

STRING: 9606.ENSP00000262144

UniGene: Hs.280951

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