WDR59 Antibody

Datasheet

Code	CSB-PA764810LA01HU
Size	US$166
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Image	Immunofluorescence staining of U251 cells with CSB-PA764810LA01HU at 1:173, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L). Western Blot Positive WB detected in: 293T whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate All lanes: WDR59 antibody at 1:2000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 110, 65, 63, 47 kDa Observed band size: 110 kDa
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) WDR59 Polyclonal antibody

Uniprot No.

Q6PJI9

Target Names

WDR59

Alternative Names

WDR59 antibody; KIAA1923 antibody; FP977 antibody; GATOR complex protein WDR59 antibody; WD repeat-containing protein 59 antibody

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human GATOR complex protein WDR59 protein (619-862AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The WDR59 Antibody (Product code: CSB-PA764810LA01HU) is Non-conjugated. For WDR59 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA764810LB01HU	WDR59 Antibody, HRP conjugated	ELISA
FITC	CSB-PA764810LC01HU	WDR59 Antibody, FITC conjugated
Biotin	CSB-PA764810LD01HU	WDR59 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:1000-1:5000
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

As a component of the GATOR subcomplex GATOR2, functions within the amino acid-sensing branch of the TORC1 signaling pathway. Indirectly activates mTORC1 and the TORC1 signaling pathway through the inhibition of the GATOR1 subcomplex. It is negatively regulated by the upstream amino acid sensors SESN2 and CASTOR1.

Subcellular Location

Lysosome membrane.

Protein Families

WD repeat WDR59 family

Database Links

HGNC: 25706

OMIM: 617418

KEGG: hsa:79726

STRING: 9606.ENSP00000262144

UniGene: Hs.280951

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Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

Related pathways

mTOR signaling pathway

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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