WTAP Antibody

Datasheet

Code	CSB-PA618970LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: Mouse kidney tissue, Rat heart tissue, Rat liver tissue All lanes: WTAP antibody at 3μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 45, 18 kDa Observed band size: 45 kDa Immunohistochemistry of paraffin-embedded human testis tissue using CSB-PA618970LA01HU at dilution of 1:100 Immunofluorescent analysis of Hela cells using CSB-PA618970LA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) WTAP Polyclonal antibody

Uniprot No.

Q15007

Target Names

WTAP

Alternative Names

DKFZp686F20131 antibody; Female-lethal(2)D homolog antibody; FL2D_HUMAN antibody; hFL(2)D antibody; KIAA0105 antibody; MGC3925 antibody; Mum2 antibody; OTTHUMP00000017522 antibody; OTTHUMP00000017523 antibody; PNAS 132 antibody; PNAS-132 antibody; PNAS132 antibody; Pre mRNA splicing regulator WTAP antibody; Pre-mRNA-splicing regulator WTAP antibody; Putative pre mRNA splicing regulator female lethal 2D homolog antibody; Putative pre mRNA splicing regulator female lethal(2D) antibody; Putative pre-mRNA splicing regulator female lethal(2D) homolog antibody; putative pre-mRNA splicing regulator female-lethal(2D) antibody; Wilms tumor 1 associated protein antibody; Wilms tumor 1 associating protein antibody; Wilms tumor 1-associating protein antibody; Wilms' tumor 1 associating protein antibody; Wilms' tumour 1-associating protein antibody; WT1 associated protein antibody; WT1-associated protein antibody; WT1-associating protein antibody; wtap antibody

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human Pre-mRNA-splicing regulator WTAP protein (1-151AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The WTAP Antibody (Product code: CSB-PA618970LA01HU) is Non-conjugated. For WTAP Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA618970LB01HU	WTAP Antibody, HRP conjugated	ELISA
FITC	CSB-PA618970LC01HU	WTAP Antibody, FITC conjugated
Biotin	CSB-PA618970LD01HU	WTAP Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:20-1:200
IF	1:50-1:200

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Associated component of the WMM complex, a complex that mediates N6-methyladenosine (m6A) methylation of RNAs, a modification that plays a role in the efficiency of mRNA splicing and RNA processing. Required for accumulation of METTL3 and METTL14 to nuclear speckle. Acts as a mRNA splicing regulator. Regulates G2/M cell-cycle transition by binding to the 3' UTR of CCNA2, which enhances its stability. Impairs WT1 DNA-binding ability and inhibits expression of WT1 target genes.

Gene References into Functions

WTAP was over-expressed in glioma tissues, and the expression was closely correlated with glioma grade. PMID: 27370540
WTAP as a regulatory subunit is required for formation of a functional m6A methyltransferase complex including METTL3 and METTL14, which play an important role in the regulation of gene expression and alternative splicing. PMID: 24407421
WTAP regulates migration and invasion of cholangiocarcinoma cells. PMID: 23354623
WTAP has an important role in abnormal proliferation and arrested differentiation of leukemia cells, and WTAP is a novel client protein of Hsp90 PMID: 24413322
the WTAP complex is a novel component of the RNA processing machinery, implying an important role in both posttranscriptional control and cell cycle regulation. PMID: 24100041
WTAP overexpression made cancer cells more tumorigenic PMID: 22957919
WTAP level in poodocytes is a valuable alternative technique in the study of podocyte injury in diabetic nephropathy. PMID: 19969384
WTAP protein involved in the morphogenesis of the genitourinaty tract and in the etiology of isolated hypospadias. PMID: 14675924
WTAP and ATF3 Transcription Factor are activated in Wilms' tumor and accelerate tumorigenesis. PMID: 16912181
WTAP as an essential factor for the stabilization of cyclin A2 mRNA, thereby regulating G2/M cell-cycle transition PMID: 17088532
WTAP is a novel regulator of the cell cycle and cell survival and implicate a WTAP-WT1 axis as a novel pathway for controlling vascular smooth muscle cell phenotype. PMID: 17095724
Nuclear degradation of Wilms tumor 1-associating protein and survivin splice variant switching underlie IGF-1-mediated survival PMID: 19605357

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Subcellular Location

Nucleus speckle. Nucleus, nucleoplasm. Cytoplasm.

Protein Families

Fl(2)d family

Tissue Specificity

Ubiquitously expressed.

Database Links

HGNC: 16846

OMIM: 605442

KEGG: hsa:9589

STRING: 9606.ENSP00000351141

UniGene: Hs.446091

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Service

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Protein Service

Gene Synthesis Service

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

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Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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