WWC3 Antibody

Code CSB-PA890929NA01HU
Size US$166
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Image
  • IHC image of CSB-PA890929NA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of U251 cells with CSB-PA890929NA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Western Blot
    Positive WB detected in: MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate
    All lanes: WWC3 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 123, 66 kDa
    Observed band size: 123 kDa

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Product Details

Full Product Name
Rabbit anti-Homo sapiens (Human) WWC3 Polyclonal antibody
Uniprot No.
Target Names
WWC3
Alternative Names
WWC3 antibody; KIAA1280 antibody; Protein WWC3 antibody
Raised in
Rabbit
Species Reactivity
Human
Immunogen
Peptide sequence from Human Protein WWC3 protein (65-85AA)
Immunogen Species
Homo sapiens (Human)
Conjugate
Non-conjugated

The WWC3 Antibody (Product code: CSB-PA890929NA01HU) is Non-conjugated. For WWC3 Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA890929NB01HU WWC3 Antibody, HRP conjugated ELISA
FITC CSB-PA890929NC01HU WWC3 Antibody, FITC conjugated
Biotin CSB-PA890929ND01HU WWC3 Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB, IHC, IF
Recommended Dilution
Application Recommended Dilution
WB 1:500-1:2000
IHC 1:20-1:200
IF 1:50-1:200
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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