IHC image of CSB-PA890929NA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.
Immunofluorescence staining of U251 cells with CSB-PA890929NA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
Positive WB detected in: MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate
All lanes: WWC3 antibody at 1:1000
Goat polyclonal to rabbit IgG at 1/50000 dilution
Predicted band size: 123, 66 kDa
Observed band size: 123 kDa