WWC3 Antibody

Datasheet
Code CSB-PA890929NA01HU
Size US$299
Uniprot No. Q9ULE0
Image
  • IHC image of CSB-PA890929NA01HU diluted at 1:100 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of U251 cells with CSB-PA890929NA01HU at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Western Blot
    Positive WB detected in: MCF-7 whole cell lysate, SH-SY5Y whole cell lysate, HepG2 whole cell lysate, 293 whole cell lysate
    All lanes: WWC3 antibody at 1:1000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 123, 66 kDa
    Observed band size: 123 kDa

Immunogen Recombinant Human Protein WWC3 protein (65-85AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:200, IF:1:50-1:200
Relevance cytosol, kinase binding, protein complex scaffold, negative regulation of hippo signaling, negative regulation of organ growth, negative regulation of transcription from RNA polymerase II promoter
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method Antigen Affinity Purified
Isotype IgG
Clonality Polyclonal
Alias Protein WWC3, WWC3, KIAA1280
Immunogen Species Homo sapiens (Human)
Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Research Area Cell Biology
Target Names WWC3
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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