ZDHHC12 Antibody

Datasheet

Code	CSB-PA822219LA01HU
Size	US$166
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Image	IHC image of CSB-PA822219LA01HU diluted at 1:50 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-rabbit polymer IgG labeled by HRP and visualized using 0.05% DAB. Western Blot Positive WB detected in: MCF7 whole cell lysate(30µg), COLO205 whole cell lysate(30µg) , HEK293 whole cell lysate(30µg), Jurkat whole cell lysate(30µg), U251 whole cell lysate(30µg), Mouse brain tissue lysate(30µg), Rat brain tissue lysate(30µg) All lanes: ZDHHC12 antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/40000 dilution Predicted band size: 31, 25, 37 kDa Observed band size: 37 kDa Exposure time: 60s
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Product Details
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ZDHHC12 Polyclonal antibody

Uniprot No.

Q96GR4

Target Names

ZDHHC12

Alternative Names

ZDHHC12; ZNF400; PSEC0008; Palmitoyltransferase ZDHHC12; DHHC domain-containing cysteine-rich protein 12; DHHC-12; Zinc finger DHHC domain-containing protein 12; Zinc finger protein 400

Raised in

Rabbit

Species Reactivity

Human, Mouse, Rat

Immunogen

Recombinant Human Probable palmitoyltransferase ZDHHC12 protein(65-140aa)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ZDHHC12 Antibody (Product code: CSB-PA822219LA01HU) is Non-conjugated. For ZDHHC12 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA822219LB01HU	ZDHHC12 Antibody, HRP conjugated	ELISA
FITC	CSB-PA822219LC01HU	ZDHHC12 Antibody, FITC conjugated
Biotin	CSB-PA822219LD01HU	ZDHHC12 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4

Form

Liquid

Tested Applications

ELISA, WB,IHC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:2000
IHC	1:20-1:100

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Customer Reviews and Q&A

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Target Background

Function

Palmitoyltransferase that could catalyze the addition of palmitate onto various protein substrates. Has a palmitoyltransferase activity toward gephyrin/GPHN, regulating its clustering at synapses and its function in gamma-aminobutyric acid receptor clustering. Thereby, indirectly regulates GABAergic synaptic transmission.

Subcellular Location

Golgi apparatus membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein.

Protein Families

DHHC palmitoyltransferase family

Tissue Specificity

Widely expressed.

Database Links

HGNC: 19159

KEGG: hsa:84885

STRING: 9606.ENSP00000361748

UniGene: Hs.133122

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Protein Service

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Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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