ZDHHC14 Antibody

Datasheet
Code CSB-PA811637LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q8IZN3
Image
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate
    All lanes: ZDHHC14 antibody at 10.5µg/ml
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 54, 52 kDa
    Observed band size: 54 kDa

  • IHC image of CSB-PA811637LA01HU diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA811637LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Probable palmitoyltransferase ZDHHC14 protein (328-453AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500
Relevance endoplasmic reticulum, palmitoyltransferase activity, protein palmitoylation
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Probable palmitoyltransferase ZDHHC14 (EC 2.3.1.225) (NEW1 domain-containing protein) (NEW1CP) (Zinc finger DHHC domain-containing protein 14) (DHHC-14), ZDHHC14
Immunogen Species Human
Research Area Cell Biology
Target Names ZDHHC14
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Subcellular Location Membrane, Multi-pass membrane protein
Protein Families DHHC palmitoyltransferase family, ERF2/ZDHHC9 subfamily
Database Links

HGNC: 20341

KEGG: hsa:79683

STRING: 9606.ENSP00000352821

UniGene: Hs.596214

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