ZDHHC14 Antibody

Datasheet

Code	CSB-PA811637LA01HU
Size	US$166
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Image	Western Blot Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate All lanes: ZDHHC14 antibody at 10.5µg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 54, 52 kDa Observed band size: 54 kDa IHC image of CSB-PA811637LA01HU diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Immunofluorescence staining of HepG2 cells with CSB-PA811637LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).
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Target Background

Product Details

Full Product Name

Rabbit anti-Homo sapiens (Human) ZDHHC14 Polyclonal antibody

Uniprot No.

Q8IZN3

Target Names

ZDHHC14

Alternative Names

ZDHHC14; Palmitoyltransferase ZDHHC14; DHHC domain-containing cysteine-rich protein 14; DHHC-14; NEW1 domain-containing protein; NEW1CP; Zinc finger DHHC domain-containing protein 14

Raised in

Rabbit

Species Reactivity

Human

Immunogen

Recombinant Human Probable palmitoyltransferase ZDHHC14 protein (328-453AA)

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

The ZDHHC14 Antibody (Product code: CSB-PA811637LA01HU) is Non-conjugated. For ZDHHC14 Antibody with conjugates, please check the following table.

Conjugate	Product Code	Product Name	Application
HRP	CSB-PA811637LB01HU	ZDHHC14 Antibody, HRP conjugated	ELISA
FITC	CSB-PA811637LC01HU	ZDHHC14 Antibody, FITC conjugated
Biotin	CSB-PA811637LD01HU	ZDHHC14 Antibody, Biotin conjugated	ELISA

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB, IHC, IF

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:500-1:1000
IF	1:200-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

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Target Background

Function

Palmitoyltransferase that could catalyze the addition of palmitate onto various protein substrates. May have a palmitoyltransferase activity toward the beta-2 adrenergic receptor/ADRB2 and thereby regulate G protein-coupled receptor signaling. May play a role in cell differentiation and apoptosis.

Gene References into Functions

Results indicate that ZDHHC14 is involved in tumor progression in patients with scirrhous type gastric cancer. PMID: 24807047
Identify ZDHHC14 as a novel tumour suppressor gene that is commonly down-regulated in testicular germ cell tumours and prostate cancer. PMID: 24407904

Subcellular Location

Endoplasmic reticulum membrane; Multi-pass membrane protein. Golgi apparatus, Golgi stack membrane; Multi-pass membrane protein.

Protein Families

DHHC palmitoyltransferase family, ERF2/ZDHHC9 subfamily

Tissue Specificity

Widely expressed.

Database Links

HGNC: 20341

KEGG: hsa:79683

STRING: 9606.ENSP00000352821

UniGene: Hs.596214

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

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