ZDHHC14 Antibody

Code CSB-PA811637LA01HU
See More Details Free Antibody trial simple
Size US$299
Uniprot No. Q8IZN3
  • Western Blot
    Positive WB detected in: HepG2 whole cell lysate, 293 whole cell lysate, Jurkat whole cell lysate, A549 whole cell lysate
    All lanes: ZDHHC14 antibody at 10.5µg/ml
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 54, 52 kDa
    Observed band size: 54 kDa

  • IHC image of CSB-PA811637LA01HU diluted at 1:600 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • Immunofluorescence staining of HepG2 cells with CSB-PA811637LA01HU at 1:200, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human Probable palmitoyltransferase ZDHHC14 protein (328-453AA)
Raised in Rabbit
Species Reactivity Human
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:5000, IHC:1:500-1:1000, IF:1:200-1:500
Relevance endoplasmic reticulum, palmitoyltransferase activity, protein palmitoylation
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias Probable palmitoyltransferase ZDHHC14 (EC (NEW1 domain-containing protein) (NEW1CP) (Zinc finger DHHC domain-containing protein 14) (DHHC-14), ZDHHC14
Immunogen Species Human
Research Area Cell Biology
Target Names ZDHHC14
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Still Have Questions? Leave a Message or Start an on-line Chat
Subcellular Location Membrane, Multi-pass membrane protein
Protein Families DHHC palmitoyltransferase family, ERF2/ZDHHC9 subfamily
Database Links

HGNC: 20341

KEGG: hsa:79683

STRING: 9606.ENSP00000352821

UniGene: Hs.596214

Related Products

ZDHHC14 Antibodies

ZDHHC14 Antibodies for Homo sapiens (Human)

ZDHHC14 Proteins

ZDHHC14 Proteins for Homo sapiens (Human)

Most popular with customers


Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.