lktA Antibody

Code CSB-PA314743EA01ESE
Size US$166
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Product Details

Full Product Name
Rabbit anti-Mannheimia haemolytica lktA Polyclonal antibody
Uniprot No.
Target Names
lktA
Alternative Names
lktA antibody; Leukotoxin antibody; Lkt antibody
Raised in
Rabbit
Species Reactivity
Mannheimia haemolytica
Immunogen
Recombinant Mannheimia haemolytica Leukotoxin protein (715-953AA)
Immunogen Species
Mannheimia haemolytica
Conjugate
Non-conjugated

The lktA Antibody (Product code: CSB-PA314743EA01ESE) is Non-conjugated. For lktA Antibody with conjugates, please check the following table.

Available Conjugates
Conjugate Product Code Product Name Application
HRP CSB-PA314743EB01ESE lktA Antibody, HRP conjugated ELISA
FITC CSB-PA314743EC01ESE lktA Antibody, FITC conjugated
Biotin CSB-PA314743ED01ESE lktA Antibody, Biotin conjugated ELISA
Clonality
Polyclonal
Isotype
IgG
Purification Method
>95%, Protein G purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Form
Liquid
Tested Applications
ELISA
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

CUSABIO produced the polyclonal antibody against lktA by immunizing rabbits with a synthetic peptide that corresponds to amino acids 715-953 of the recombinant Leukotoxin protein. The antiserum obtained from the rabbits was purified using protein G affinity chromatography, resulting in a purity level of 95% for the anti-lktA antibody.

This antibody is an unconjugated IgG that specifically reacts with Escherichia coli lktA. LktA is a virulence factor generated by the bacterium Mannheimia haemolytica, which targets and kills immune cells, particularly leukocytes, in the lungs of infected animals. The lktA polyclonal antibody developed by CUSABIO is suitable for detecting the lktA protein in ELISA.

Customer Reviews and Q&A

 Customer Reviews
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Applications : WB

Sample type: goats and lambs

Review: Mannheimia haemolytica (Mha) culture growth and Lkt secretion profile. (a): SDS page of Mha culture supernatant samples. (b): Anti-Lkt Western blot of Mha culture supernatant samples. SM: Molecular weight marker(color protein standard broad range P7712S Biolabs).

By Anonymous

Target Background

Function
Pasteurella leukotoxins are exotoxins that attack host leukocytes and especially polymorphonuclear cells, by causing cell rupture. The leukotoxin binds to the host LFA-1 integrin and induces a signaling cascade leading to many biological effects, including tyrosine phosphorylation of the CD18 tail, elevation of the intracellular Ca(2+) and lysis of the host cell. This leukotoxin is a major contributor to the pathogenesis of lung injury in ovine pneumonic pasteurellosis. It has also weak hemolytic activity.
Subcellular Location
Secreted. Host cell membrane; Multi-pass membrane protein.
Protein Families
RTX prokaryotic toxin (TC 1.C.11) family
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