Recombinant 5'-AMP-activated protein kinase catalytic subunit alpha-2 (aak-2), partial

Code CSB-YP839238CXY
MSDS
Size Pls inquire
Source Yeast
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP839238CXY
MSDS
Size Pls inquire
Source E.coli
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-EP839238CXY-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-BP839238CXY
MSDS
Size Pls inquire
Source Baculovirus
Have Questions? Leave a Message or Start an on-line Chat
Code CSB-MP839238CXY
MSDS
Size Pls inquire
Source Mammalian cell
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Purity
>85% (SDS-PAGE)
Target Names
aak-2
Uniprot No.
Alternative Names
aak-2; T01C8.15'-AMP-activated protein kinase catalytic subunit alpha-2; AMPK subunit alpha-2; EC 2.7.11.1
Species
Caenorhabditis elegans
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Target Background

Function
Acts as a sensor that couples lifespan to information about energy levels and insulin-like signals. Role in motility and response to oxidative stress. Involved in the establishment of germline stem cell (GSC) quiescence during dauer development. Plays a role in axon regrowth after axotomy in PLM neurons. Plays a role in the maintenance of glycogen stores which are necessary for resistance to hyperosmotic stress. Plays a role in the regulation of flp-7 secretion from ASI neurons. Keeps the CREB-regulated transcription coactivator 1 homolog crtc-1 inactive which in turn inhibits flp-7 secretion. Following serotonin signaling, derepresses crtc-1 which stimulates flp-7 secretion and subsequent body fat loss.
Gene References into Functions
  1. Knockdown of Indy/CeNAC2 increased life span by 22+/-3 % compared to controls, together with a decrease in whole body fat content by ~50%. Indy/CeNAC2 reduction also increased the activation of the intracellular energy sensor AMPK/aak2. PMID: 26318988
  2. As in elevated serotonin signaling, inactivation of AAK-2 in the ASI neurons caused enhanced secretion of dense core vesicles from these neurons. PMID: 24921650
  3. An essential output of RSKS-1 in axon regrowth is the metabolic sensor AMP kinase, AAK-2. PMID: 24431434
  4. AMPK catalytic subunit aak-2 mutants exhibited elevated pharyngeal pumping even in the absence of exogenous 5-HT. PMID: 22768843
  5. The capacity for an animal to survive long bouts of severe oxygen deprivation is likely dependent on specific subunits of the heterotrimeric protein AMPK and energy stores such as carbohydrates. PMID: 21304820
  6. These results show that aging is controlled by overlapping but distinct pathways and that AMPK/aak-2 represents a node in a network of evolutionarily conserved biochemical pathways that control aging. PMID: 16626391
  7. C. elegans larvae that lack LKB1/AMPK (AMP-activated protein kinase) signalling enter dauer normally, but then rapidly consume their stored energy and prematurely expire following vital organ failure PMID: 19052547
  8. aak-2 is required for life span extension in states of impaired glycolysis and calorie restriction. aak-2 initiates increased mitochondrial respiration and increased oxidative stress/ROS formation to promote mitohormesis. PMID: 17908557

Show More

Hide All

Protein Families
Protein kinase superfamily, CAMK Ser/Thr protein kinase family, SNF1 subfamily
Tissue Specificity
Expressed in the pharynx, the ventral cord, neurons including the hermaphrodite-specific neuron, body wall muscles, the vulva, the excretory canal, and weakly in the intestine.
Database Links

KEGG: cel:CELE_T01C8.1

STRING: 6239.T01C8.1b

UniGene: Cel.17479

icon of phone
Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
icon of address
Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
icon of social media
Join us with

Subscribe newsletter

Leave a message

* To protect against spam, please pass the CAPTCHA test below.
CAPTCHA verification
© 2007-2024 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1