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Recombinant Akkermansia muciniphila Crossover junction endodeoxyribonuclease RuvC (ruvC)

Recombinant Akkermansia muciniphila Crossover junction endodeoxyribonuclease RuvC (ruvC)

^{In Stock}

Code	CSB-EP453002AZF
Abbreviation	Recombinant Akkermansia muciniphila ruvC protein
MSDS	MSDS Datasheet
Size	$388
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP453002AZF could indicate that this peptide derived from E.coli-expressed Akkermansia muciniphila (strain ATCC BAA-835 / Muc) ruvC. Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP453002AZF could indicate that this peptide derived from E.coli-expressed Akkermansia muciniphila (strain ATCC BAA-835 / Muc) ruvC.
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Product Details
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Target Background

Product Details

Purity

Greater than 85% as determined by SDS-PAGE.

Target Names

ruvC

Uniprot No.

B2UP63

Research Area

Epigenetics and Nuclear Signaling

Alternative Names

ruvC; Amuc_2024; Crossover junction endodeoxyribonuclease RuvC; EC 3.1.22.4; Holliday junction nuclease RuvC; Holliday junction resolvase RuvC

Species

Akkermansia muciniphila (strain ATCC BAA-835 / Muc)

Source

E.coli

Expression Region

1-167aa

Target Protein Sequence

MRILAIDPAIRNTGYAVVEGDYRRARALDYGTLSIPRSVSQSGCLLAIKQHLGNLIDKWNPDEMAVERIIYVQSHQTAITMGAAKAAVVIAAAEAGLRIMEYSPKSVKLSVVGRGAAQKTQVAFMVRALLELRETPESDAADALAIGLTHLFSADPLKAHMMERKYI
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.

Mol. Weight

23.2 kDa

Protein Length

Full Length

Tag Info

N-terminal 10xHis-tagged and C-terminal Myc-tagged

Form

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

3-7 business days

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Explore the role of Crossover junction endodeoxyribonuclease RuvC with Recombinant Akkermansia muciniphila (strain ATCC BAA-835 / Muc) ruvC protein. This full-length protein, covering the 1-167aa expression region, is sourced from E. coli, facilitating cost-effective production. The N-terminal 10xHis-tag and C-terminal Myc-tag enable efficient purification and detection, respectively. With a purity of greater than 85% as determined by SDS-PAGE, the lyophilized recombinant Akkermansia muciniphila ruvC protein offers a valuable resource for researchers focusing on epigenetics and nuclear signaling in the context of this important gut microbiome species.

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Target Background

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group.

Protein Families

RuvC family

Database Links

KEGG: amu:Amuc_2024

STRING: 349741.Amuc_2024

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