Recombinant Arabidopsis thaliana 5'-3' exoribonuclease 2 (XRN2), partial

Code CSB-YP875525DOA
MSDS
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Source Yeast
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Code CSB-EP875525DOA
MSDS
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Source E.coli
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Code CSB-EP875525DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP875525DOA
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Source Baculovirus
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Code CSB-MP875525DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
XRN2
Uniprot No.
Alternative Names
XRN2; At5g42540/At5g42550; K16E1.1/K16E1.2; 5'-3' exoribonuclease 2; AtXRN2; EC 3.1.13.-; Protein EXORIBONUCLEASE 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Possesses 5'->3' exoribonuclease activity. Acts as an endogenous post-transcriptional gene silencing (PTGS) suppressor. Degrades miRNA-derived loops, excised during miRNA maturation in the nucleus. Involved in pre-rRNA processing. Involved in the primary exonucleolytic shortening of the 5' external transcribed spacer (5'ETS), required for endonucleolytic processing at site P by the U3 snoRNP complex. Involved with XRN3 in the 5'-end processing of 5.8S and 25S rRNAs. Contributes with XRN3 to polyadenylation-dependent nuclear RNA surveillance. Involved in the degradation of aberrant polyadenylated pre-rRNA through 5'-end processing.
Gene References into Functions
  1. AtXRN2 has a role in ribosomal RNA processing. PMID: 20338880
  2. The nuclear exoribonucleases XRN2 and XRN3 the nucleotidase/phosphatase FIERY1 are endogenous posttranscriptional gene silencing suppressors. PMID: 17993620
Subcellular Location
Nucleus.
Protein Families
5'-3' exonuclease family, XRN2/RAT1 subfamily
Tissue Specificity
Expressed in roots, leaves, stems and flowers.
Database Links

KEGG: ath:AT5G42540

STRING: 3702.AT5G42540.2

UniGene: At.16936

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