Recombinant Arabidopsis thaliana ADP-ribosylation factor GTPase-activating protein AGD10 (AGD10)

Code CSB-YP525749DOA
MSDS
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Source Yeast
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Code CSB-EP525749DOA
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Source E.coli
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Code CSB-EP525749DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP525749DOA
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Source Baculovirus
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Code CSB-MP525749DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AGD10
Uniprot No.
Alternative Names
AGD10; MEE28; RPA; At2g35210; T4C15.12ADP-ribosylation factor GTPase-activating protein AGD10; ARF GAP AGD10; Protein ARF-GAP DOMAIN 10; AtAGD10; Protein MATERNAL EFFECT EMBRYO ARREST 28; Protein ROOT AND POLLEN ARFGAP
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-395
Target Protein Sequence
MASENLNDKI SVFKKLKAKS DNKICFDCNA KNPTWASVTY GIFLCIDCSA VHRSLGVHIS FVRSTNLDSW SSEQLKMMIY GGNNRAQVFF KQYGWSDGGK TEAKYTSRAA DLYKQILAKE VAKSKAEEEL DLPPSPPDST QVPNGLSSIK TSEALKESNT LKQQEKPDVV PVSPRISRSV KKPLGAKKTG KTGGLGARKL TTKSSGTLYD QKPEESVIIQ ATSPVSAKSA RSSFSSRFDY ADNVQNREDY MSPQVVSHVA PPKSSGFFEE ELEMNGGRFQ KKPITSSSKL QIQETDEARK KFTNAKSISS AQYFGNDNNS ADLEAKSSLK KFSGSSAISS ADLFGDGDGD FPLDLTAGDL LNRLSLQAQQ DISSLKNMAE ETKKKLGSVA SSLWV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
GTPase-activating protein (GAP) for ADP ribosylation factor (ARF). Activates ARF1 and ARF2. Required for female gametophyte development. Involved in root hair and pollen tube growth.
Gene References into Functions
  1. The findings support a model in which IDN2 interacts with RPA and facilitates the release of RPA from single-stranded DNA tails and subsequent recruitment of RAD51 at DSB sites to promote DSB repair. PMID: 28223440
  2. plays a role in root hair and pollen tube growth, most likely through the regulation of Arabidopsis ARF1 and ARF1-like protein U5 activity PMID: 16731582
  3. AtRPA1a is required for class I crossover formation but is dispensable for meiotic DNA break repair PMID: 19153602
Subcellular Location
Golgi apparatus.
Tissue Specificity
Expressed specifically in roots, pollen grains and pollen tubes.
Database Links

KEGG: ath:AT2G35210

STRING: 3702.AT2G35210.1

UniGene: At.37696

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