Recombinant Arabidopsis thaliana ATP-dependent DNA helicase Q-like 4A (RECQL4A), partial

Code CSB-YP810545DOA
MSDS
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Source Yeast
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Code CSB-EP810545DOA
MSDS
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Source E.coli
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Code CSB-EP810545DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP810545DOA
MSDS
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Source Baculovirus
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Code CSB-MP810545DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
RECQL4A
Uniprot No.
Alternative Names
RECQL4A; RECQ4A; RQL4A; SGS1; At1g10930; T19D16.15; ATP-dependent DNA helicase Q-like 4A; EC 3.6.4.12; RecQ-like protein 4A; AtRecQ4A; AtRecQl4A; SGS1-like protein; AtSGS1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
3'-5' DNA helicase involved in DNA repair. Required for the maintenance of genome stability by modulation of the DNA damage response and repression of crossovers. Confers resistance to genotoxic stress. Suppresses spontaneous homologous recombination (HR) events in somatic cells together with its partners RMI1 and TOP3A. Contributes to the maintenance of chromosome integrity during meiosis. Involved in the removal of telomeric bridges that appear to arise during meiotic recombination. Required to resolve or dissolve MSH4-dependent telomeric associations. Does not seem required for chiasma formation.
Gene References into Functions
  1. The N-terminus of RECQ4A was determined to be required for its specific functions in DNA repair and recombination. PMID: 24174542
  2. Pollen viability decreased in Atrecq4A, resulting in a reduction in fertility.Immunolocalization confirmed the association of AtRECQ4A with the telomeres during prophase I. PMID: 21265901
  3. RECQ4A and MUS81 define RAD5A-independent pathways of DNA repair. RAD5A and RECQ4A act through two independent pathways for intrastrand cross-link DNA repair. RECQ4A and MUS81 act in parallel pathways to resolve replication-associated DNA intermediates. PMID: 20971895
  4. Roles for Arabidopsis RecQl4A in maintenance of genome stability by modulation of the DNA damage response and suppression of HR are reported. PMID: 16146519
Subcellular Location
Nucleus. Chromosome.
Protein Families
Helicase family, RecQ subfamily
Tissue Specificity
Expressed in roots, seedlings, young leaves, shoots, shoot apical mersitem, inflorescences, flowers, siliques and seeds.
Database Links

KEGG: ath:AT1G10930

STRING: 3702.AT1G10930.1

UniGene: At.10177

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