Recombinant Arabidopsis thaliana Actin-related protein 6 (ARP6)

Code CSB-YP818595DOA
MSDS
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Source Yeast
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Code CSB-EP818595DOA
MSDS
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Source E.coli
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Code CSB-EP818595DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP818595DOA
MSDS
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Source Baculovirus
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Code CSB-MP818595DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARP6
Uniprot No.
Alternative Names
ARP6; ESD1; SUF3; At3g33520; T4P3.8Actin-related protein 6; Protein EARLY IN SHORT DAYS 1; Protein SUPPRESSOR OF FRIGIDA 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-421
Target Protein Sequence
MSNIVVLDNG GGLIKAGQGG ERDPTTVIPN CLYKPLSSKK FIHPSPLTTL SDEIDLTSAA VRRPIDRGYL INSDLQREIW SHLFTSLLHI APSSSSLLLT EAPLSIPSVQ RTTDELVFED FGFSSLYIAH PQSLVHLYEA SRQPDSILSK TQCSLVVDCG FSFTHAVPVL HNFTLNHAIK RIDLGGKAFT NYLKELVSYR SINVMDETFL VDDAKEKLCF VSLDLLRDLR LARNGNTLIK STYVLPDGVT HTKGYVKDPQ AAKRFLSLSE KESVVVMDKV GERKKADMNK NEIDLTNERF LVPETLFQPA DLGMNQAGLA ECIVRAINSC HSYLQPVLYQ SIILTGGSTL FPQLKERLEG ELRPLVPDHF DVKITTQEDP ILGVWRGGSL LASSPDFESM CVTKAEYEEL GSARCRRRFF H
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant H2A.F/Z leading to transcriptional regulation of selected genes (e.g. FLC) by chromatin remodeling. Binds to the promoter region of FLC chromatin. Required for the activation of FLC and FLC/MAF genes expression to levels that inhibit flowering, through both histone H3 and H4 acetylation and methylation mechanisms. Involved in several developmental processes including organization of plant organs, leaves formation, flowering time repression, and fertility. Modulates photoperiod-dependent epidermal leaves cell development; promotes cell division in long days, and cell expansion/division in short days. May be involved in the regulation of pathogenesis-related proteins (PRs).
Gene References into Functions
  1. ARP6 is required for proper H2A.Z deposition at a number of Pi starvation response (PSR) genes in Arabidopsis. [ARP6] PMID: 19897606
  2. ARP6 acts in the nucleus to regulate plant development, and it does so through modulation of chromatin structure and the control of gene expression.[AtARP6] PMID: 16141450
  3. ESD1 encodes ATARP6, a putative component of chromatin remodelling that positively regulates FLC accumulation. PMID: 16495307
Subcellular Location
Nucleus. Cytoplasm. Note=Localized in the nucleus during the interphase, but is released into the cytoplasm during the mitotic phase (PubMed:16141450). Associated to heterochromatin (PubMed:16141450). Localized at the nuclear periphery when interacting with SWC6 (PubMed:16155178, PubMed:17470967).
Protein Families
Actin family, ARP6 subfamily
Tissue Specificity
Mostly expressed in flowers, and, to a lower extent, in seedlings, shoot apex, stems, siliques, seeds, and roots (at protein level).
Database Links

KEGG: ath:AT3G33520

STRING: 3702.AT3G33520.1

UniGene: At.18479

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