Recombinant Arabidopsis thaliana Adenosylhomocysteinase 1 (SAHH1)

Code CSB-YP522805DOA
MSDS
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Source Yeast
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Code CSB-EP522805DOA
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Source E.coli
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Code CSB-EP522805DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP522805DOA
MSDS
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Source Baculovirus
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Code CSB-MP522805DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SAHH1
Uniprot No.
Alternative Names
SAHH1; EMB1395; HOG1; At4g13940; dl3010w; Adenosylhomocysteinase 1; AdoHcyase 1; EC 3.3.1.1; Protein EMBRYO DEFECTIVE 1395; Protein HOMOLOGY-DEPENDENT GENE SILENCING 1; S-adenosyl-L-homocysteine hydrolase 1; SAH hydrolase 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-485
Target Protein Sequence
MALLVEKTSS GREYKVKDMS QADFGRLELE LAEVEMPGLM ACRTEFGPSQ PFKGARITGS LHMTIQTAVL IETLTALGAE VRWCSCNIFS TQDHAAAAIA RDSAAVFAWK GETLQEYWWC TERALDWGPG GGPDLIVDDG GDATLLIHEG VKAEEIFEKT GQVPDPTSTD NPEFQIVLSI IKEGLQVDPK KYHKMKERLV GVSEETTTGV KRLYQMQQNG TLLFPAINVN DSVTKSKFDN LYGCRHSLPD GLMRATDVMI AGKVAVICGY GDVGKGCAAA MKTAGARVIV TEIDPICALQ ALMEGLQVLT LEDVVSEADI FVTTTGNKDI IMVDHMRKMK NNAIVCNIGH FDNEIDMLGL ETYPGVKRIT IKPQTDRWVF PETKAGIIVL AEGRLMNLGC ATGHPSFVMS CSFTNQVIAQ LELWNEKASG KYEKKVYVLP KHLDEKVALL HLGKLGARLT KLSKDQSDYV SIPIEGPYKP PHYRY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Adenosylhomocysteine is a competitive inhibitor of S-adenosyl-L-methionine-dependent methyl transferase reactions; therefore adenosylhomocysteinase may play a key role in the control of methylations via regulation of the intracellular concentration of adenosylhomocysteine. Required for DNA methylation-dependent gene silencing.
Gene References into Functions
  1. SAHH1 plays a critical role in methyl homeostasis, and its deficiency is a major contributing factor to the change of global gene expression, metabolic pathways and activation of transposable elements in the sahh1 mutant. PMID: 22555436
  2. A histone methylation-dependent DNA methylation pathway is uniquely impaired by deficiency in adenosylhomocysteinase. PMID: 16951055
  3. HOG1 has high affinity cytokinin binding activity. Overexpression led to early flowering with a significantly reduced plant biomass and number of leaves. PMID: 18592247
Protein Families
Adenosylhomocysteinase family
Database Links

KEGG: ath:AT4G13940

STRING: 3702.AT4G13940.1

UniGene: At.24056

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