Recombinant Arabidopsis thaliana Alpha-humulene/ (-)- (E)-beta-caryophyllene synthase (TPS21), partial

Code CSB-YP768018DOA
MSDS
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Source Yeast
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Code CSB-EP768018DOA
MSDS
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Source E.coli
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Code CSB-EP768018DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP768018DOA
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Source Baculovirus
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Code CSB-MP768018DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
TPS21
Uniprot No.
Alternative Names
TPS21; PUP8; At5g23960; MZF18.16; Alpha-humulene/(-)-(E)-beta-caryophyllene synthase; EC 4.2.3.-; Terpenoid synthase 21; AtTPS21
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Involved in sesquiterpene (C15) biosynthesis. The major products are beta-caryophyllene and alpha-humulene. Does not convert geranyl diphosphate (GPP) to any monoterpenes.
Gene References into Functions
  1. MYC2 directly binds to promoter of the sesquiterpene synthase gene TPS21 PMID: 22669881
  2. The florally expressed genes At5g23960 and At5g44630, are responsible for the formation of virtually all sesquiterpenes found in the Arabidopsis floral volatile blend. PMID: 15918888
  3. Data show that the enzyme activity of an herbivore-induced A. lyrata ssp. petraea terpene synthase with high similarity to A. thaliana TPS 21. PMID: 19322583
Subcellular Location
Cytoplasm.
Protein Families
Terpene synthase family, Tpsa subfamily
Tissue Specificity
Expressed exclusively in flowers. Expressed in the flower stigmata and also detected in the mesocarp cell layers of the silique wall.
Database Links

KEGG: ath:AT5G23960

STRING: 3702.AT5G23960.1

UniGene: At.30985

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