Product Details

Purity

>85% (SDS-PAGE)

Target Names

PIN3

Uniprot No.

Q9S7Z8

Alternative Names

PIN3; At1g70940; F15H11.14; Auxin efflux carrier component 3; AtPIN3

Species

Arabidopsis thaliana (Mouse-ear cress)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Acts as a component of the auxin efflux carrier. Seems to be involved in the lateral auxin transport system and mediates tropic growth. Coordinated polar localization of PIN3 is directly regulated by the vesicle trafficking process.

Gene References into Functions

Two mechanistically distinct PIN3 polarization events redirect auxin fluxes at different time points of the gravity response: first, gravity-mediated redirection of PIN3-mediated auxin flow toward the lower hypocotyl side, where auxin gradually accumulates and promotes growth, and later PIN3 polarization to the opposite cell side, depleting this auxin maximum to end the bending. PMID: 27773568
Functional interplay between protein kinase CK2 and salicylic acid sustains PIN3 transcriptional expression. PMID: 24547808
asymmetric distribution of auxin is essential for root negative phototropism, and is regulated by blue-light-induced PIN3 polarization PMID: 23888933
Sequential induction of auxin efflux (PIN3) and influx (LAX3) carriers regulates lateral root emergence in Arabidopsis. PMID: 24150423
Arabidopsis thaliana PIN3 auxin transporter is required for the asymmetric auxin distribution for the gravitropic response. PMID: 21569134
The phosphorylation code of PIN3-hydrophilic loop is operational for the determination of PIN3 polarity in the Arabidopsis guard cell. PMID: 22902703
The loss of PIN3 causes a dramatic delay in the onset of Lateral root initiation (LRI) after founder cell (FC) are specified, which can be fully recovered by the expression of PIN3 exclusively in the endodermis. PMID: 23178590
PIN3-mediated auxin gradients participate in pulse-induced phototropism. PMID: 22843667
Mutation of phosphorylation sites caused defects in PIN3-mediated biological processes such as auxin efflux activity, auxin maxima formation, root growth, and root gravitropism. PMID: 22519832
Data show that light polarizes the cellular localization of the auxin efflux carrier PIN3 in hypocotyl endodermis cells, resulting in changes in auxin distribution and differential growth. PMID: 21394084
Data show that pin3 mutants are outcompeted by wild-type neighbors who suppress fitness of pin3-3 by 40%. PMID: 21149713
Cold stress blocks the early phase of gravity response by inhibiting the lateral relocalization of PIN3. PMID: 20040541
NOV is required for cell type-specific expression of PIN3 in the root. PMID: 19880797
findings show that both ARL2 and ARG1 function in a gravity signal transduction pathway with PIN3, an auxin efflux facilitator that is expressed in the statocytes PMID: 18047472

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Subcellular Location

Cell membrane; Multi-pass membrane protein.

Protein Families

Auxin efflux carrier (TC 2.A.69.1) family

Tissue Specificity

Predominantly expressed at the lateral side of shoot endodermis cells as well as root pericycle and columella cells.

Database Links

KEGG: ath:AT1G70940

STRING: 3702.AT1G70940.1

UniGene: At.11573

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Recombinant Arabidopsis thaliana Auxin efflux carrier component 3 (PIN3), partial

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