Recombinant Arabidopsis thaliana COP9 signalosome complex subunit 8 (CSN8)

Code CSB-YP005796DOA
MSDS
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Source Yeast
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Code CSB-EP005796DOA
MSDS
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Source E.coli
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Code CSB-EP005796DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP005796DOA
MSDS
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Source Baculovirus
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Code CSB-MP005796DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CSN8
Uniprot No.
Alternative Names
CSN8; COP9; FUS7; At4g14110; dl3095cCOP9 signalosome complex subunit 8; CSN complex subunit 8; Constitutive photomorphogenesis protein 9; Protein FUSCA 7
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-197
Target Protein Sequence
MDLSPVKEAL AAKSFDKIAD ICDTLMLQVA SEGIEYHDDW PYAIHLLGYF YVDDCDSARF LWKRIPTAIK ERKPEVVAAW GIGQKLWTHD YAGVYEAIRG YDWSQEAKDM VAAFSDLYTK RMFQLLLSAY STITIHDLAL FLGMTEDDAT TYVVENGWTV DAASQMASVK KQAVKREQKV DSSKLQRLTE YVFHLEH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of the COP9 signalosome complex (CSN), a complex involved in various cellular and developmental processes such as photomorphogenesis and auxin and jasmonate responses. The CSN complex is an essential regulator of the ubiquitin (Ubl) conjugation pathway by mediating the deneddylation of the cullin subunits of SCF-type E3 ligase complexes, leading to decrease the Ubl ligase activity of SCF. It is involved in repression of photomorphogenesis in darkness by regulating the activity of COP1-containing Ubl ligase complexes. The complex is also required for degradation of IAA6 by regulating the activity of the Ubl ligase SCF-TIR complex.
Gene References into Functions
  1. while constitutively photomorphogenic9 (COP9) signalosome (CSN) mutants can complete embryogenesis and are able to germinate, they progressively lose meristem activity upon germination until they become unable to sustain growth PMID: 26277260
  2. This review provides an overview of the highly complex regulation of CULLIN-RING E3 ubiquitin ligase activity by COP9 signalosome (CSN), and the many roles of the CSN in plant development and defense. PMID: 22325866
  3. SMAP1 interacts with the COP9 signalosome. PMID: 22576848
  4. COP9 has a role in ethylene signaling. PMID: 18429939
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
CSN8 family
Tissue Specificity
Ubiquitous.
Database Links

KEGG: ath:AT4G14110

STRING: 3702.AT4G14110.1

UniGene: At.4252

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