Product Details

Purity

>85% (SDS-PAGE)

Target Names

CBL1

Uniprot No.

O81445

Alternative Names

CBL1; SCABP5; At4g17615; dl4845w; FCAALL.122Calcineurin B-like protein 1; SOS3-like calcium-binding protein 5

Species

Arabidopsis thaliana (Mouse-ear cress)

Expression Region

2-213

Target Protein Sequence

GCFHSKAAK EFRGHEDPVK LASETAFSVS EVEALFELFK SISSSVVDDG LINKEEFQLA LFKSRKRENI FANRIFDMFD VKRKGVIDFG DFVRSLNVFH PNASLEDKID FTFRLYDMDC TGYIERQEVK QMLIALLCES EMKLADETIE IILDKTFEDA DVNQDGKIDK LEWSDFVNKN PSLLKIMTLP YLRDITTTFP SFVFHSEVDE IAT

Protein Length

Full Length of Mature Protein

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Acts as a calcium sensor involved in the signaling pathway during growth and development and in response to abiotic stresses. May function as a positive regulator of salt and drought responses and as a negative regulator of cold response. Contributes to the regulation of early stress-related CBF/DREB transcription factors. CBL proteins interact with CIPK serine-threonine protein kinases. Binding of a CBL protein to the regulatory NAF domain of a CIPK protein lead to the activation of the kinase in a calcium-dependent manner. Mediates the activation of AKT1 by CIPK proteins (CIPK6, CIPK16, and CIPK23) in response to low potassium conditions and in the context of stomatal movement. Involved in response to glucose and gibberellin during germination and seedling development and in response to cold stress. Involved in the calcium-dependent regulation by CIPK26 of reactive oxygen species production by the NADPH oxidase RBOHF.

Gene References into Functions

These analyses reveal not only the fundamental role of CBL1 in the adjustment of central transcriptomic networks involved in maintaining adequate physiological homeostasis processes, but also that a high shoot-root dynamics is required for the proper deployment of Al resistance responses in the root. PMID: 28150888
a crucial function for CBL1 and CBL9 in pollen germination and tube growth PMID: 23741064
These results indicate a novel role for CBL1 in modulating responses to glucose and gibberellic acid signals. PMID: 23437128
a novel general regulatory mechanism of CBL-CIPK complexes in that CBL phosphorylation at their flexible C terminus likely provokes conformational changes that enhance specificity and activity of CBL-CIPK complexes toward their target proteins PMID: 22253446
Deletion of CBL1 may disturb the perception of calcium influx by cold stress and results in altered cold-induced signaling and adaption. PMID: 21600398
The results of this study imply that plasma membrane-localized CBL1- and CBL9-CIPK23 complexes simultaneously regulate potassium transport processes in roots and in stomatal guard cells. PMID: 17922773
Results show that a 12-amino acid peptide of CBL1 is sufficient to mediate dual lipid modification and to confer plasma membrane targeting. PMID: 18502848

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Subcellular Location

Cell membrane; Lipid-anchor. Note=The cell membrane localization is S-acylation dependent and is abolished by 2-bromopalmitate (2-BP) treatment.

Protein Families

Calcineurin regulatory subunit family

Tissue Specificity

Preferentially expressed in stems, roots and siliques. Coexpressed with CIPK15/PKS3 in guard cells. Co-localized with CIPK23 in root tips and vascular bundles in the stem and the leaf, as well as in guard cells.

Database Links

KEGG: ath:AT4G17615

STRING: 3702.AT4G17615.1

UniGene: At.11088

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Recombinant Arabidopsis thaliana Calcineurin B-like protein 1 (CBL1)

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