Recombinant Arabidopsis thaliana Cell division protein FtsY homolog, chloroplastic (CPFTSY)

Code CSB-YP529291DOA
MSDS
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Source Yeast
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Code CSB-EP529291DOA
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Source E.coli
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Code CSB-EP529291DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP529291DOA
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Source Baculovirus
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Code CSB-MP529291DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CPFTSY
Uniprot No.
Alternative Names
CPFTSY; FTSY; At2g45770; F4I18.25Cell division protein FtsY homolog; chloroplastic; Chloroplast SRP receptor homolog; alpha subunit CpFtsY; Fused signal recognition particle receptor
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
41-366
Target Protein Sequence
CSAGPSGFFT RLGRLIKEKA KSDVEKVFSG FSKTRENLAV IDELLLFWNL AETDRVLDEL EEALLVSDFG PKITVRIVER LREDIMSGKL KSGSEIKDAL KESVLEMLAK KNSKTELQLG FRKPAVIMIV GVNGGGKTTS LGKLAHRLKN EGTKVLMAAG DTFRAAASDQ LEIWAERTGC EIVVAEGDKA KAATVLSKAV KRGKEEGYDV VLCDTSGRLH TNYSLMEELI ACKKAVGKIV SGAPNEILLV LDGNTGLNML PQAREFNEVV GITGLILTKL DGSARGGCVV SVVEELGIPV KFIGVGEAVE DLQPFDPEAF VNAIFS
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Signal recognition particle receptor protein. Binds GTP specifically. The GTPase activity is inhibited by the N-terminus of the protein until binding to the thylakoid membrane. Activates the GTPase activity of FFC/cpSRP54 when bound to the cpSRP complex. Required for light-harvesting chlorophyll a/b-binding protein (LHCP) integration into thylakoids. Might be also functionally linked to the Sec translocation machinery.
Gene References into Functions
  1. Mutations within the cpFtsY gene are associated with an inability to induce Fe(III) chelate reductase activity in response to iron deficiency. PMID: 16813577
  2. These results highlight intriguing differences between the classical and chloroplast SRP and SRP receptor GTPases, and help explain how the chloroplast SRP pathway can mediate efficient targeting of proteins to the thylakoid membrane. PMID: 17475780
  3. the crystal structure of chloroplast FtsY (cpFtsY) at 1.75 A resolution. PMID: 18022392
  4. cpftsY mutants generally exhibit defects in the biogenesis of light-harvesting chlorophyll proteins and other thylakoidal membrane proteins that are less severe than in the alb3 mutants. PMID: 18764927
  5. cpFtsY membrane-binding motif plays a critical role in the intramolecular communication that regulates cpSRP receptor functions at the membrane. PMID: 19293157

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Subcellular Location
Plastid, chloroplast stroma. Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side. Note=The membrane-binding domain is capable of partial insertion into the lipid bilayer.
Protein Families
GTP-binding SRP family
Tissue Specificity
Expressed in green tissues. Low levels in roots and seeds.
Database Links

KEGG: ath:AT2G45770

STRING: 3702.AT2G45770.2

UniGene: At.491

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