Recombinant Arabidopsis thaliana Chaperone protein ClpC1, chloroplastic (CLPC1), partial

Code CSB-YP863761DOA
MSDS
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Source Yeast
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Code CSB-EP863761DOA
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Source E.coli
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Code CSB-EP863761DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP863761DOA
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Source Baculovirus
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Code CSB-MP863761DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CLPC1
Uniprot No.
Alternative Names
CLPC1; DCA1; HSP93-V; IRM1; At5g50920; K3K7.7; Chaperone protein ClpC1; chloroplastic; ATP-dependent Clp protease ATP-binding subunit ClpC homolog 1; Casein lytic proteinase C1; Protein DE-REGULATED CAO ACCUMULATION 1; Protein IRON-RESCUED MUTANT 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Molecular chaperone that hydrolyzes ATP and is associated with the chloroplast protein import apparatus. May function as the motor for chloroplast protein translocation, as translocation requires ATP hydrolysis in the stroma. May interact with a ClpP-like protease involved in degradation of denatured proteins in the chloroplast. Involved in the regulation of chlorophyll b biosynthesis through the destabilization of chlorophyllide a oxygenase (CAO) protein in response to the accumulation of chlorophyll b. Involved in leaf iron homeostasis.
Gene References into Functions
  1. Data show that the loop connecting the two halves of AtClpD N-terminal domain (NTD) is longer and covers the region which in case of AtClpC1 is thought to contribute to adaptor protein interaction. PMID: 28287170
  2. indicate that CLPC1 plays a direct or indirect role in chloroplast transcriptome homeostasis PMID: 30208840
  3. Hsp93 role in protein import and proteolysis and association with chloroplast envelope. PMID: 26586836
  4. that of the two near-identical ClpC paralogs (ClpC1 and ClpC2) in Arabidopsis chloroplasts, along with the closely related ClpD, it is ClpC1 that is the most abundant throughout leaf maturation. PMID: 24599948
  5. ClpC1 is involved in leaf Fe homeostasis, presumably via chloroplast translocation of some nuclear-encoded proteins which function in Fe transport. PMID: 20382967
  6. data suggest that overall ClpC as a stromal molecular chaperone plays a vital role in chloroplast function and leaf development and is likely involved in photosystem biogenesis. [clpC1] PMID: 15563614
  7. Study indicated that a chloroplast Clp protease is involved in regulating chlorophyll b biosynthesis through the destabilization of chlorophyllide a oxygenase protein in response to the accumulation of chlorophyll b. PMID: 17291312
  8. cpSRP54 deletion led in young leaves to up-regulation of thylakoid proteases and stromal chaperones, including ClpC. PMID: 18633119

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Subcellular Location
Plastid, chloroplast stroma. Plastid, chloroplast membrane.
Protein Families
ClpA/ClpB family, ClpC subfamily
Tissue Specificity
Highly expressed in rosette leaves. Expressed in roots, stems and inflorescences. Expressed in photosynthetic green tissues with high levels in young, developing leaf tissues.
Database Links

KEGG: ath:AT5G50920

STRING: 3702.AT5G50920.1

UniGene: At.24774

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