Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) CLPC1 Polyclonal antibody

Uniprot No.

Q9FI56

Target Names

CLPC1

Alternative Names

CLPC1 antibody; DCA1 antibody; HSP93-V antibody; IRM1 antibody; At5g50920 antibody; K3K7.7 antibody; Chaperone protein ClpC1 antibody; chloroplastic antibody; ATP-dependent Clp protease ATP-binding subunit ClpC homolog 1 antibody; Casein lytic proteinase C1 antibody; Protein DE-REGULATED CAO ACCUMULATION 1 antibody; Protein IRON-RESCUED MUTANT 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana CLPC1 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Molecular chaperone that hydrolyzes ATP and is associated with the chloroplast protein import apparatus. May function as the motor for chloroplast protein translocation, as translocation requires ATP hydrolysis in the stroma. May interact with a ClpP-like protease involved in degradation of denatured proteins in the chloroplast. Involved in the regulation of chlorophyll b biosynthesis through the destabilization of chlorophyllide a oxygenase (CAO) protein in response to the accumulation of chlorophyll b. Involved in leaf iron homeostasis.

Gene References into Functions

Data show that the loop connecting the two halves of AtClpD N-terminal domain (NTD) is longer and covers the region which in case of AtClpC1 is thought to contribute to adaptor protein interaction. PMID: 28287170
indicate that CLPC1 plays a direct or indirect role in chloroplast transcriptome homeostasis PMID: 30208840
Hsp93 role in protein import and proteolysis and association with chloroplast envelope. PMID: 26586836
that of the two near-identical ClpC paralogs (ClpC1 and ClpC2) in Arabidopsis chloroplasts, along with the closely related ClpD, it is ClpC1 that is the most abundant throughout leaf maturation. PMID: 24599948
ClpC1 is involved in leaf Fe homeostasis, presumably via chloroplast translocation of some nuclear-encoded proteins which function in Fe transport. PMID: 20382967
data suggest that overall ClpC as a stromal molecular chaperone plays a vital role in chloroplast function and leaf development and is likely involved in photosystem biogenesis. [clpC1] PMID: 15563614
Study indicated that a chloroplast Clp protease is involved in regulating chlorophyll b biosynthesis through the destabilization of chlorophyllide a oxygenase protein in response to the accumulation of chlorophyll b. PMID: 17291312
cpSRP54 deletion led in young leaves to up-regulation of thylakoid proteases and stromal chaperones, including ClpC. PMID: 18633119

Hide All

Subcellular Location

Plastid, chloroplast stroma. Plastid, chloroplast membrane.

Protein Families

ClpA/ClpB family, ClpC subfamily

Tissue Specificity

Highly expressed in rosette leaves. Expressed in roots, stems and inflorescences. Expressed in photosynthetic green tissues with high levels in young, developing leaf tissues.

Database Links

KEGG: ath:AT5G50920

STRING: 3702.AT5G50920.1

UniGene: At.24774

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CLPC1 Antibody

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