Recombinant Arabidopsis thaliana Chlorophyllase-1 (CLH1)

Code CSB-EP519505DOA
Abbreviation Recombinant Mouse-ear cress CLH1 protein
MSDS
Size $388
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  • (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
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Product Details

Purity
Greater than 90% as determined by SDS-PAGE.
Activity
Not Test
Target Names
CLH1
Uniprot No.
Research Area
Others
Alternative Names
AtCLH1;Chlorophyll-chlorophyllido hydrolase 1;Chlase 1;Coronatine-induced protein 1;CORI1
Species
Arabidopsis thaliana (Mouse-ear cress)
Source
E.coli
Expression Region
1-324aa
Target Protein Sequence
MAAIEDSPTFSSVVTPAAFEIGSLPTTEIPVDPVENDSTAPPKPVRITCPTVAGTYPVVLFFHGFYLRNYFYSDVLNHIASHGYILVAPQLCKLLPPGGQVEVDDAGSVINWASENLKAHLPTSVNANGKYTSLVGHSRGGKTAFAVALGHAATLDPSITFSALIGIDPVAGTNKYIRTDPHILTYKPESFELDIPVAVVGTGLGPKWNNVMPPCAPTDLNHEEFYKECKATKAHFVAADYGHMDMLDDDLPGFVGFMAGCMCKNGQRKKSEMRSFVGGIVVAFLKYSLWGEKAEIRLIVKDPSVSPAKLDPSPELEEASGIFV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
Mol. Weight
41.8 kDa
Protein Length
Full Length
Tag Info
C-terminal 6xHis-tagged
Form
Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA
Please contact us to get it.

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Target Background

Function
Catalyzes the hydrolysis of ester bond in chlorophyll to yield chlorophyllide and phytol. Shows a preferential activity toward chlorophyll a. Does not seem to be required for chlorophyll degradation during senescence. May modulate the balance between different plant defense pathways.
Gene References into Functions
  1. These results support the presence of a unique binary defense system against insect herbivores involving chlorophyll and CLH. PMID: 25583926
  2. Data conclude that AtCLH1 might be required at the initial stage of leaf senescence, quite likely playing a role in converting Chl b to a. PMID: 18349515
Subcellular Location
Cytoplasm, cytosol.
Protein Families
AB hydrolase superfamily, Lipase family
Tissue Specificity
Expressed in seedlings, leaves, flowers and siliques, but not in roots.
Database Links

KEGG: ath:AT1G19670

STRING: 3702.AT1G19670.1

UniGene: At.19779

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