Recombinant Arabidopsis thaliana Chlorophyllase-1 (CLH1)

Code CSB-YP519505DOA
MSDS
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Source Yeast
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Code CSB-EP519505DOA
MSDS
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Source E.coli
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Code CSB-EP519505DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP519505DOA
MSDS
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Source Baculovirus
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Code CSB-MP519505DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CLH1
Uniprot No.
Alternative Names
CLH1; ATHCOR1; COR1; At1g19670; F6F9.28Chlorophyllase-1; AtCLH1; EC 3.1.1.14; Chlorophyll-chlorophyllido hydrolase 1; Chlase 1; Coronatine-induced protein 1; CORI1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-324
Target Protein Sequence
MAAIEDSPTF SSVVTPAAFE IGSLPTTEIP VDPVENDSTA PPKPVRITCP TVAGTYPVVL FFHGFYLRNY FYSDVLNHIA SHGYILVAPQ LCKLLPPGGQ VEVDDAGSVI NWASENLKAH LPTSVNANGK YTSLVGHSRG GKTAFAVALG HAATLDPSIT FSALIGIDPV AGTNKYIRTD PHILTYKPES FELDIPVAVV GTGLGPKWNN VMPPCAPTDL NHEEFYKECK ATKAHFVAAD YGHMDMLDDD LPGFVGFMAG CMCKNGQRKK SEMRSFVGGI VVAFLKYSLW GEKAEIRLIV KDPSVSPAKL DPSPELEEAS GIFV
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Catalyzes the hydrolysis of ester bond in chlorophyll to yield chlorophyllide and phytol. Shows a preferential activity toward chlorophyll a. Does not seem to be required for chlorophyll degradation during senescence. May modulate the balance between different plant defense pathways.
Gene References into Functions
  1. These results support the presence of a unique binary defense system against insect herbivores involving chlorophyll and CLH. PMID: 25583926
  2. Data conclude that AtCLH1 might be required at the initial stage of leaf senescence, quite likely playing a role in converting Chl b to a. PMID: 18349515
Subcellular Location
Cytoplasm, cytosol.
Protein Families
AB hydrolase superfamily, Lipase family
Tissue Specificity
Expressed in seedlings, leaves, flowers and siliques, but not in roots.
Database Links

KEGG: ath:AT1G19670

STRING: 3702.AT1G19670.1

UniGene: At.19779

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