Recombinant Arabidopsis thaliana Cyclin-dependent kinase inhibitor 1 (KRP1)

Code CSB-YP721194DOA
MSDS
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Source Yeast
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Code CSB-EP721194DOA
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Source E.coli
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Code CSB-EP721194DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP721194DOA
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Source Baculovirus
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Code CSB-MP721194DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
KRP1
Uniprot No.
Alternative Names
KRP1; ICK1; At2g23430; F26B6.8Cyclin-dependent kinase inhibitor 1; Inhibitor/interactor of CDK protein 1; KIP-related protein 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-191
Target Protein Sequence
MVRKYRKAKG IVEAGVSSTY MQLRSRRIVY VRSEKSSSVS VVGDNGVSSS CSGSNEYKKK ELIHLEEEDK DGDTETSTYR RGTKRKLFEN LREEEKEELS KSMENYSSEF ESAVKESLDC CCSGRKTMEE TVTAEEEEKA KLMTEMPTES EIEDFFVEAE KQLKEKFKKK YNFDFEKEKP LEGRYEWVKL E
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Binds and inhibits CYCD2-1/CDKA-1 kinase complex activity. Regulates cell division which is crucial for plant growth, development and morphogenesis. Functions in turning cells from a mitotic to an endoreplicating cell cycle mode. Acts cell- and non-cell-autonomously to regulate endoreduplication by allowing S phase progression, but blocking entry into mitosis. Keeps on the one hand the plant cell cycle locally controlled, and on the other hand provides a possibility of linking cell cycle control in single cells with the supracellular organization of a tissue or an organ. May target specifically CDKA-1.
Gene References into Functions
  1. A short motif in ICK1 plays role in ICK degradation and stability PMID: 27233081
  2. ICK1 and ICK5 may be involved in developmental delay and that the suppressed growth under glucose treatment probably resulted from disturbed mitotic and endoreduplication cycle in autotetraploid Arabidopsis. PMID: 22476290
  3. This work demonstrates that impeding cell cycle progression by means of ICK1/KRP1 and ICK2/KRP2 overexpression severely restricts gall development. PMID: 23574394
  4. Arabidopsis ICK1 blocks entry into mitosis but allows S-phase progression causing endoreplication, suggesting that plant CKIs act in a concentration-dependent manner and have an important function in cell proliferation as well as in cell cycle exit. PMID: 15749764
  5. ICK1/KRP1 localizes to different subnuclear domains, and is an unstable protein with its degradation mediated by at least two domains. PMID: 16766674
  6. Nuclear localization of ICK1 is regulated and suggests a possible role of ICK1 in regulating the cellular distribution of CDKA. PMID: 16845478
  7. The role of KRP1 in the plant cell cycle, and its degradation pathways, are reported. PMID: 18005227

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Subcellular Location
Nucleus, nucleoplasm. Note=Distributed in a ponctuate pattern and in heterochromatic chromocenters.
Protein Families
CDI family, ICK/KRP subfamily
Tissue Specificity
Expressed at low levels in roots, stems, leaves and flowers.
Database Links

KEGG: ath:AT2G23430

STRING: 3702.AT2G23430.1

UniGene: At.24577

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