Recombinant Arabidopsis thaliana Cysteine protease ATG4a(ATG4A)

Code CSB-YP837318DOA
Size Pls inquire other sizes
Source Yeast
Code CSB-EP837318DOA
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Source E.coli
Code CSB-EP837318DOA-B
Size Pls inquire other sizes
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
Code CSB-BP837318DOA
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Source Baculovirus
Code CSB-MP837318DOA
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Source Mammalian cell

Product Details

Purity >85% (SDS-PAGE)
Target Names ATG4A
Uniprot No. Q8S929
Species Arabidopsis thaliana (Mouse-ear cress)
Expression Region 1-467
Target Protein Sequence MKALCDRFVP QQCSSSSKSD THDKSPLVSD SGPSDNKSKF TLWSNVFTSS SSVSQPYRES STSGHKQVCT TRNGWTAFVK RVSMASGAIR RFQERVLGPN RTGLPSTTSD VWLLGVCYKI SADENSGETD TGTVLAALQL DFSSKILMTY RKGFEPFRDT TYTSDVNWGC MIRSSQMLFA QALLFHRLGR AWTKKSELPE QEYLETLEPF GDSEPSAFSI HNLIIAGASY GLAAGSWVGP YAICRAWESL ACKKRKQTDS KNQTLPMAVH IVSGSEDGER GGAPILCIED ATKSCLEFSK GQSEWTPIIL LVPLVLGLDS VNPRYIPSLV ATFTFPQSVG ILGGKPGAST YIVGVQEDKG FYLDPHEVQQ VVTVNKETPD VDTSSYHCNV LRYVPLESLD PSLALGFYCR DKDDFDDFCL RALKLAEESN GAPLFTVTQT HTAINQSNYG FADDDSEDER EDDWQML
Protein Length full length protein
Tag Info The following tags are available.
N-terminal His-tagged
Tag-Free
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form Lyophilized powder
Buffer before Lyophilization Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting
and FAQs
Protein FAQs
Storage Condition Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet Please contact us to get it.
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Target Data

Function Cysteine protease required for autophagy, which is able to cleave the C-terminal part of ATG8 that may be subsequently converted to a smaller form, with a revealed C-terminal glycine. ATG8 is then activated by ATG7 and is subsequently attached to ATG3, another E2-like enzyme, after which the C-terminal glycine of ATG8 is conjugated to phosphatidylethanolamine by an amide bond. This conjugated form has the capacity for the binding to autophagosomes.
Subcellular Location Cytoplasm
Protein Families Peptidase C54 family
Tissue Specificity Contitutively expressed.
Database Links

KEGG: ath:AT2G44140

STRING: 3702.AT2G44140.1

UniGene: At.48588

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