Recombinant Arabidopsis thaliana Cytochrome P450 86B1 (CYP86B1), partial

Code CSB-YP887818DOA1
MSDS
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Source Yeast
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Code CSB-EP887818DOA1
MSDS
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Source E.coli
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Code CSB-EP887818DOA1-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP887818DOA1
MSDS
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Source Baculovirus
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Code CSB-MP887818DOA1
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
CYP86B1
Uniprot No.
Alternative Names
CYP86B1; At5g23190; MKD15.5; Cytochrome P450 86B1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in very long chain fatty acids (VLCFA) omega-hydroxylation. Required for the synthesis of saturated VLCFA alpha, omega-bifunctional suberin monomers.
Gene References into Functions
  1. CYP86B1 is a very long chain fatty acid hydroxylase specifically involved in polyester monomer biosynthesis during the course of plant development. PMID: 19525321
Subcellular Location
Endoplasmic reticulum membrane; Single-pass membrane protein. Note=In vitro import assays suggest an association with the outer chloroplastic membrane; also detected in the chloroplasts of guard cells when expressed in a heterologous system.
Protein Families
Cytochrome P450 family
Tissue Specificity
Expressed in roots endodermis, anthers, stigmas, stomata of young pedicels of inflorescences, the placenta region of siliques, at the level of the hilum in matures seeds, at the junction of siliques to pedicels where abscission of floral parts takes place
Database Links

KEGG: ath:AT5G23190

STRING: 3702.AT5G23190.1

UniGene: At.8800

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