Recombinant Arabidopsis thaliana Dehydration-responsive element-binding protein 1C (DREB1C)

Code CSB-YP871211DOA
MSDS
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Source Yeast
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Code CSB-EP871211DOA
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Source E.coli
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Code CSB-EP871211DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP871211DOA
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Source Baculovirus
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Code CSB-MP871211DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
DREB1C
Uniprot No.
Alternative Names
DREB1C; CBF2; ERF030; At4g25470; M7J2.161; Dehydration-responsive element-binding protein 1C; Protein DREB1C; C-repeat/dehydration-responsive element-binding factor 2; C-repeat-binding factor 2; CRT/DRE-binding factor 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-216
Target Protein Sequence
MNSFSAFSEM FGSDYESPVS SGGDYSPKLA TSCPKKPAGR KKFRETRHPI YRGVRQRNSG KWVCELREPN KKTRIWLGTF QTAEMAARAH DVAAIALRGR SACLNFADSA WRLRIPESTC AKEIQKAAAE AALNFQDEMC HMTTDAHGLD MEETLVEAIY TPEQSQDAFY MDEEAMLGMS SLLDNMAEGM LLPSPSVQWN YNFDVEGDDD VSLWSY
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]CCGAC-3'. Binding to the C-repeat/DRE element mediates cold-inducible transcription. CBF/DREB1 factors play a key role in freezing tolerance and cold acclimation.
Gene References into Functions
  1. This study reveals the essential functions of C-repeat binding factors (CBF) in chilling stress response and cold acclimation, as well as defines a set of genes as CBF regulon. PMID: 27353960
  2. CBFs play an important role in the trade-off between cold tolerance and plant growth through the precise regulation of COR genes in the complicated transcriptional network. PMID: 28009483
  3. the three CBF genes together are required for cold acclimation and freezing tolerance. PMID: 27252305
  4. Data indicate that the C-repeat binding factor (CBF) locus includes three genes - CBF1, CBF2 and CBF3 (AT4G25480) - that are induced by low temperature and encode transcription factors. PMID: 26369909
  5. A major locus harboring three cold-responsive transcription factor genes CBF1, was identified. PMID: 23721132
  6. Studies indicate that DREB1A (CBF3), DREB1B (CBF1) and DREB1C (CBF2) play an important role in increasing stress tolerance. PMID: 23271026
  7. Transcriptional changes in cell wall metabolism-related components under overexpression of a key transcription factor for freezing tolerance, CBF2, were identified. PMID: 21611181
  8. Overexpression of CBF2 in Arabidopsis suppressed the responsiveness of leaf tissues to ethylene compared with that in wild-type plants, as manifested in significantly delayed senescence and chlorophyll degradation. PMID: 20636906
  9. These results indicate that overexpression of CBF2 not only increases frost tolerance, but also affects other developmental processes. PMID: 19854800
  10. SOC1 Directly Represses the Expression of CBF2 Genes. PMID: 19825833
  11. PIF7 functions as a transcriptional repressor for DREB1C expression and its activity is regulated by PIF7-interacting factors TIMING OF CAB EXPRESSION1 and Phytochrome B. PMID: 19837816
  12. We explored the regulation of CBF1-3 by the circadian clock. PMID: 15728337
  13. The low freezing tolerance of FTQ4-Cvi (FREEZING TOLERANCE QTL 4) alleles was associated with a deletion of the promoter region of Cvi CBF2, and with low RNA expression of CBF2 and of several CBF target genes. PMID: 16244146
  14. CBF1 and CBF3, but not CBF2 have a concerted additive effect to induce the whole CBF regulon and the complete development of cold acclimation PMID: 18093929
  15. Important evolutionary changes in CBF1, -2, and -3 may have primarily occurred at the level of gene regulation as well as in protein function. PMID: 18990244

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Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, ERF subfamily
Tissue Specificity
Expressed in leaves and roots.
Database Links

KEGG: ath:AT4G25470

STRING: 3702.AT4G25470.1

UniGene: At.233

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