Recombinant Arabidopsis thaliana Glutamate receptor 3.4 (GLR3.4), partial

Code CSB-YP812752DOA
MSDS
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Source Yeast
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Code CSB-EP812752DOA
MSDS
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Source E.coli
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Code CSB-EP812752DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP812752DOA
MSDS
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Source Baculovirus
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Code CSB-MP812752DOA
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
GLR3.4
Uniprot No.
Alternative Names
GLR3.4; GLR4; GLUR3; At1g05200; YUP8H12.19Glutamate receptor 3.4; AtGLR4; Ligand-gated ion channel 3.4
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Glutamate-gated receptor that probably acts as non-selective cation channel, at least in hypocotyls (Probable). Can be triggered by Asn, Ser, Gly and, to a lower extent, Ala, Cys and Glu. May be involved in light-signal transduction and calcium homeostasis via the regulation of calcium influx into cells (Probable). Plays an important role in the calcium-based fast transmission of environmental stress. Acts as negative regulator of lateral root initiation and development. May restrict primordia numbers and position along the root axis by a signaling process originating in the phloem. AtGLR3.4-mediated cytosolic calcium influx may be involved in the regulation of seed germination under salt stress by modulating sodium accumulation through the SOS pathway.
Gene References into Functions
  1. AtGLR3.4-mediated Ca2+ influx may be involved in the regulation of seed germination under salt stress. PMID: 29432559
  2. AtGLR3.4 in particular and GLRs in general could play an important role in the Ca(2+) -based, fast transmission of environmental stress. PMID: 15864638
  3. The effect of 6 amino acids on the glutamate receptors of A. thaliana, and the role of GLR3.3 and GLR3.4 in the channel mechanisms are reported. PMID: 18162597
Subcellular Location
Cell membrane; Multi-pass membrane protein. Plastid, chloroplast membrane; Multi-pass membrane protein.
Protein Families
Glutamate-gated ion channel (TC 1.A.10.1) family
Tissue Specificity
Highly expressed in roots and at lower levels in leaves and siliques. Expressed in seedlings, cotyledons, roots (e.g. root hairs, epidermis and cortex cells), stems, leaves (e.g. vascular bundles and hydathodes), and siliques. Expressed in root phloem.
Database Links

KEGG: ath:AT1G05200

STRING: 3702.AT1G05200.1

UniGene: At.10412

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