Recombinant Arabidopsis thaliana Helicase protein MOM1 (MOM1), partial

Code CSB-YP888925DOA
MSDS
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Source Yeast
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Code CSB-EP888925DOA
MSDS
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Source E.coli
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Code CSB-EP888925DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP888925DOA
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Source Baculovirus
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Code CSB-MP888925DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
MOM1
Uniprot No.
Alternative Names
MOM1; MOM; At1g08060; T6D22.14; Helicase protein MOM1; EC 3.6.4.-; Protein MAINTENANCE OF METHYLATION; Protein MORPHEUS MOLECULE 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for the heritable maintenance of transcriptional gene silencing (TGS) in heterochromatin, and particularly in the intermediate bivalent heterochromatin, characterized by an unsual methylation pattern consisting in hypermethylated DNA and histone H3 'Lys-4' methylation (H3K4me) together with depletion of histone H3 'Lys-9' methylation (H3K9me), in a chromatin methylation-independent manner, in a non-CG methylation context. May play a role in the RNA polymerase IV/V (Pol-IV/V)-mediated RNA-directed DNA methylation (RdDM) leading to TGS (also called siRNA-mediated TGS pathway), probably by modulating small interfering RNA (siRNA) accumulation. Especially involved in the gene silencing of the transcriptionally silent information region (TSI), 5S ribosomal RNA genes (localized in the pericentromeric heterochromatin of chromosomes 3, 4, and 5) and of 180-bp satellite repeats and 106B long terminal repeat (LTR)-like repeats of the chromocenters. Prevents the aberrant mRNA transcriptional read-through.
Gene References into Functions
  1. synergistic transcriptional regulation in the mom1/atmorc6 double mutant, indicating that these epigenetic regulators act mainly in different silencing pathways, both independently of DNA methylation PMID: 24799676
  2. These results reveal a surprising separation of epigenetic activities that enable the single, large MOM1 protein to coordinate cooperating mechanisms of epigenetic regulation PMID: 22346760
  3. the degree of contribution of MOM1 to maintenance of the silent states varies in different loci, and other silencing machineries, including those in the RNA-directed DNA methylation pathway, interact genetically with MOM1 PMID: 21464619
  4. MOM1 plays a role in preventing aberrant RNA transcriptional read-through. PMID: 20826701
  5. Crystals of Morpheus' molecule 1 belonged to the trigonal space group P3(1)21 (or P3(2)21), with unit-cell parameters a=85.64, c=292.74 A. Structure determination is ongoing PMID: 20693667
  6. Pol-V is required for MOM1-mediated suppression of transcription at a subset of its chromosomal targets. PMID: 19910926
  7. Less than 13% of MOM1 sequence is necessary and sufficient for its transcriptional gene silencing function. PMID: 18725928

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Subcellular Location
Nucleus.
Database Links

KEGG: ath:AT1G08060

STRING: 3702.AT1G08060.1

UniGene: At.20225

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