Recombinant Arabidopsis thaliana Histone deacetylase 19 (HDA19), partial

Code CSB-YP521242DOA
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Source Yeast
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Code CSB-EP521242DOA
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Source E.coli
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Code CSB-EP521242DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP521242DOA
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Source Baculovirus
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Code CSB-MP521242DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
HDA19
Uniprot No.
Alternative Names
HDA19; HD1; HDA1; RPD3A; At4g38130; F20D10.250; Histone deacetylase 19; AtHD1; HD; EC 3.5.1.98
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-501
Target Protein Sequence
MDTGGNSLAS GPDGVKRKVC YFYDPEVGNY YYGQGHPMKP HRIRMTHALL AHYGLLQHMQ VLKPFPARDR DLCRFHADDY VSFLRSITPE TQQDQIRQLK RFNVGEDCPV FDGLYSFCQT YAGGSVGGSV KLNHGLCDIA INWAGGLHHA KKCEASGFCY VNDIVLAILE LLKQHERVLY VDIDIHHGDG VEEAFYATDR VMTVSFHKFG DYFPGTGHIQ DIGYGSGKYY SLNVPLDDGI DDESYHLLFK PIMGKVMEIF RPGAVVLQCG ADSLSGDRLG CFNLSIKGHA ECVKFMRSFN VPLLLLGGGG YTIRNVARCW CYETGVALGV EVEDKMPEHE YYEYFGPDYT LHVAPSNMEN KNSRQMLEEI RNDLLHNLSK LQHAPSVPFQ ERPPDTETPE VDEDQEDGDK RWDPDSDMDV DDDRKPIPSR VKREAVEPDT KDKDGLKGIM ERGKGCEVEV DESGSTKVTG VNPVGVEEAS VKMEEEGTNK GGAEQAFPPK T
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. HDA19 is involved in jasmonic acid and ethylene signaling of pathogen response. Part of a repressor complex including APETALA2 (AP2) and TOPLESS (TPL) that control the expression domains of numerous floral organ identity genes. Involved in negative regulation of salinity stress response. Represses the expression of stress tolerance-related genes, genes coding for late embryogenesis abundant (LEA) proteins that prevent protein aggregation, and positive regulators of abscisic acid (ABA) signaling, such as ABI5 and NAC019.
Gene References into Functions
  1. These results reveal the presence of an MSI1-HDA19 complex that fine-tunes abscissic acid signaling in Arabidopsis. PMID: 26704384
  2. SCL15 acts as an HDA19-associated regulator to repress embryonic traits in seedlings. PMID: 26129778
  3. HDC1 is a ubiquitously expressed nuclear protein that interacts with at least two deacetylases (HDA6 and HDA19), promotes histone deacetylation, and attenuates derepression of genes under water stress. PMID: 24058159
  4. HDA19 plays a negative role in basal defense mediated by the salicylic acid-dependent signaling pathway. PMID: 22381007
  5. HDA6 and HDA19 may play a redundant role in modulating seed germination and salt stress response, as well as ABA- and salt stress-induced gene expression in Arabidopsis.[HDA19] PMID: 20930557
  6. HDA19 may regulate gene expression involved in jasmonic acid and ethylene signaling of pathogen response in Arabidopsis.[HDA19] PMID: 15749761
  7. Data suggest that AtHD1 is a nuclear protein and possesses histone deacetylase activities responsible for global transcriptional regulation important to plant growth and development. PMID: 16699543
  8. These results suggest that during germination in Arabidopsis, HDA6 and HDA19 redundantly regulate the repression of embryonic properties directly or indirectly via repression of embryo-specific gene function. PMID: 18024558
  9. Disruption of HDA19 leads to compromised resistance, whereas its overexpression results in enhanced resistance to P. syringae. PMID: 18776063

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Subcellular Location
Nucleus. Note=excluded from the nucleolus, but associated with the condensing chromatids.
Protein Families
Histone deacetylase family, HD type 1 subfamily
Tissue Specificity
Highly expressed in leaves, stems, flowers and young siliques.
Database Links

KEGG: ath:AT4G38130

STRING: 3702.AT4G38130.1

UniGene: At.25069

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